Triton X-100: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(illustration) |
No edit summary |
||
| (7 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
[[Image:200px-Triton X-100.png|thumb|200px|Structure of Triton X-100]] | [[Image:200px-Triton X-100.png|thumb|200px|Structure of Triton X-100]] | ||
'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis. | |||
== Usage == | |||
* Recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf] | |||
* Keep at room temperature protected from bright light | |||
* Susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. | |||
* Addition of [[EDTA]] and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated hydroxytoluene per 500 moles of detergent to prevent autooxidation. | |||
** [[User:Etienne Robillard|Etienne Robillard]] 09:25, 24 January 2013 (EST) Hence, adding EDTA to Triton-X100 may also be used for preventing Mg2+ dependent (ie: NMDA) ligand oxidation. Thus proper precautions must be taken when handling Triton X-100/EDTA reagents. (safety google, gas mask, etc) | |||
== Tips == | |||
from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]: | |||
* The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm | |||
has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100 | |||
* When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this | |||
form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case. | |||
== See also == | == See also == | ||
| Line 10: | Line 22: | ||
* .. as part of miniprep - [[Miniprep/Qiagen kit]] | * .. as part of miniprep - [[Miniprep/Qiagen kit]] | ||
* .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]] | * .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]] | ||
* [[Critical micelle concentration (CMC)]] | |||
== External links == | == External links == | ||
Revision as of 07:25, 24 January 2013
Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
Usage
- Recommended shelf life: 2 years [1]
- Keep at room temperature protected from bright light
- Susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
- Addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated hydroxytoluene per 500 moles of detergent to prevent autooxidation.
- Etienne Robillard 09:25, 24 January 2013 (EST) Hence, adding EDTA to Triton-X100 may also be used for preventing Mg2+ dependent (ie: NMDA) ligand oxidation. Thus proper precautions must be taken when handling Triton X-100/EDTA reagents. (safety google, gas mask, etc)
Tips
from the Pierce instructions manual [2]:
- The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm
has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100
- When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this
form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.
See also
- Triton in protein solubilisation - Drummond:Solubility, Knight:Protein solubility
- .. in antibody washing/blocking - Silver:Immunofluorescence
- .. as part of miniprep - Miniprep/Qiagen kit
- .. in cell lysis and protein solubilisation for Western blot - Silver: Lysate for Western
- Critical micelle concentration (CMC)
