Triton X-100
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'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis. | '''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis. | ||
| - | == | + | == Usage == |
| - | * | + | * Recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf] |
| - | + | * Keep at room temperature protected from bright light | |
| - | + | * Susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. | |
| - | + | * Addition of [[EDTA]] and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated hydroxytoluene per 500 moles of detergent to prevent autooxidation. | |
| - | * | + | ** [[User:Etienne Robillard|Etienne Robillard]] 09:25, 24 January 2013 (EST) Hence, adding EDTA to Triton-X100 may also be used for preventing Mg2+ dependent (ie: NMDA) ligand oxidation. Thus proper precautions must be taken when handling Triton X-100/EDTA reagents. (safety google, gas mask, etc) |
| - | * | + | |
| - | * | + | |
| - | hydroxytoluene per 500 moles of detergent to prevent | + | |
== Tips == | == Tips == | ||
| Line 25: | Line 22: | ||
* .. as part of miniprep - [[Miniprep/Qiagen kit]] | * .. as part of miniprep - [[Miniprep/Qiagen kit]] | ||
* .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]] | * .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]] | ||
| + | * [[Critical micelle concentration (CMC)]] | ||
== External links == | == External links == | ||
Current revision
Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
Contents |
Usage
- Recommended shelf life: 2 years [1]
- Keep at room temperature protected from bright light
- Susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
- Addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated hydroxytoluene per 500 moles of detergent to prevent autooxidation.
- Etienne Robillard 09:25, 24 January 2013 (EST) Hence, adding EDTA to Triton-X100 may also be used for preventing Mg2+ dependent (ie: NMDA) ligand oxidation. Thus proper precautions must be taken when handling Triton X-100/EDTA reagents. (safety google, gas mask, etc)
Tips
from the Pierce instructions manual [2]:
- The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm
has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100
- When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this
form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.
See also
- Triton in protein solubilisation - Drummond:Solubility, Knight:Protein solubility
- .. in antibody washing/blocking - Silver:Immunofluorescence
- .. as part of miniprep - Miniprep/Qiagen kit
- .. in cell lysis and protein solubilisation for Western blot - Silver: Lysate for Western
- Critical micelle concentration (CMC)
