Triton X-100: Difference between revisions

Revision as of 11:36, 14 October 2008

Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.

from the Pierce instructions manual [2]:

store at room temperature protected from bright light
susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated

hydroxytoluene per 500 moles of detergent to prevent autoxidation

from the Pierce instructions manual [3]:

The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm

has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100

When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this

form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.

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 == Storage/Stability ==
+* recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf]
 from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
 * store at room temperature protected from bright light
 * susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
 * addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
 hydroxytoluene per 500 moles of detergent to prevent autoxidation
 == Tips ==