Triton X-100
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Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
Storage/Stability
from the Pierce instructions manual [1]:
- store at room temperature protected from bright light
- susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A
- addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
hydroxytoluene per 500 moles of detergent to prevent autoxidation
See also
- Triton in protein solubilisation - Drummond:Solubility, Knight:Protein solubility
- .. in antibody washing/blocking - Silver:Immunofluorescence
- .. as part of miniprep - Miniprep/Qiagen kit
- .. in cell lysis and protein solubilisation for Western blot - Silver: Lysate for Western