Turner:CV

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Education Harvard University Program in Biological and Biomedical Sciences Ph.D., Microbiology and Molecular Genetics (May 2012) Dissertation: Bistability in Pseudomonas aeruginosa University of Iowa B.S., Microbiology with Honors and Computer Science (May 2006) Research Experience The University of Texas at Austin Postdoctoral Fellow (2012-present) Advisor: Marvin Whiteley Project: Fitness determinants of P. aeruginosa for growth in disease conditions Investigating the genetic requirements for P. aeruginosa growth in CF lung secretions Map the conditionally essential genome for burn wound infection and systemic invasion Project: Mapping all synthetic genetic interactions in a bacterium using second-generation sequencing Harvard University Ph.D. Student (2006-2012) Advisor: Simon L. Dove Project: An epigenetic switch that controls virulence gene expression in P. aeruginosa Characterized the bistable BexR regulon in P. aeruginosa Determined a requirement for positive feedback in bistable expression of bexR and the bexR regulon Project: Control of cupA fimbrial gene expression in P. aeruginosa by the LysR-type transcription regulator MexT Discovered a role for PrrA, an unannotated sRNA encoded downstream of the MexT-regulated mexEFoprN antibiotic efflux pump genes, in bistable cupA gene expression Generated evidence suggesting that PrrA negatively regulates the H-NS-like protein MvaU Project: Inducible protein degradation in P. aeruginosa Adapted a targeted protein depletion system for use in P. aeruginosa and demonstrated its utility in determining the co-essentiality of the H-NS-like proteins MvaT and MvaU University of Iowa Research Assistant (2002-2006) Advisor: Michael A. Apicella Project: Biochemistry of sialic acid transport in Haemophilus influenzae Purified the sialic acid transport protein SiaP and characterized its sugar-binding biochemistry Peer-Reviewed Publications Google Scholar profile Keith H Turner, Heather R McManus, Seth R Goldman, Kirsty A McFarland, Isabelle Vallet-Gely, Bryce E Nickels, and Simon L Dove (2014). Silencing by a H-NS family member is antagonized by a small RNA in Pseudomonas aeruginosa. Submitted. Keith H Turner, Jake Everett, Urvish Trivedi, Kendra P Rumbaugh, and Marvin Whiteley (2014). Requirements for Pseudomonas aeruginosa chronic and acute wound infection. PLoS Genet: In revision. Peter Jorth, Keith H Turner, Pinar Gumus, Nejat Nizam, Nurcan Buduneli, and Marvin Whiteley (2014). Metatranscriptomics of the human oral microbiome during health and disease. mBio 5(2): e01012-14. PubMed mBio PDF Justine L Murray, Jodi L Connell, Apollo Stacy, Keith H Turner*, and Marvin Whiteley* (2014). Mechanisms of synergy in polymicrobial infections. J Microbiol 52(3): 188-99. PubMed J Microbiol PDF *Co-corresponding authors Deepak Balasubramanian, Hansi Kumari, Melita Jaric, Mitch Fernandez, Keith H Turner, Simon L Dove, Giri Narasimhan, Stephen Lory, and Kalai Mathee (2014). Deep sequencing analyses expands the Pseudomonas aeruginosa AmpR regulon to include small RNA-mediated regulation of iron acquisition, heat-shock and the oxidative stress response. Nucleic Acids Res 42(2): 979-98. PubMed Nucleic Acids Res PDF Alan Basset, Keith H Turner, Elizabeth Boush, Sabina Sayeed, Simon L Dove, and Richard Malley (2012). An epigenetic switch mediates bistable expression of the type I pilus genes in Streptococcus pneumoniae. J Bacteriol 194(5): 1088-91. PubMed J Bacteriol PDF Alan Basset, Keith H Turner, Elizabeth Boush, Sabina Sayeed, Simon L Dove, and Richard Malley (2011). Expression of the type I pneumococcal pilus is bistable and negatively regulated by the structural component RrgA. Infect Immun 79(8): 2974-83. PubMed Infect Immun Cover PDF Keith H Turner, Isabelle Vallet-Gely, and Simon L Dove (2009). Epigenetic control of virulence gene expression in Pseudomonas aeruginosa by a LysR-type transcription regulator. PLoS Genet 5(12): e1000779. PubMed PLoS Genet PDF Sandra Castang, Heather R McManus, Keith H Turner, and Simon L Dove (2008). H-NS family members function coordinately in an opportunistic pathogen. Proc Natl Acad Sci USA 105(48): 18947-52. PubMed Proc Natl Acad Sci USA PDF Jason W Johnston, Nathan P Coussens, Simon Allen, Jon CD Houtman, Keith H Turner, Anthony Zaleski, S Ramaswamy, Bradford W Gibson, and Michael A Apicella (2008). Characterization of the N-acetyl-neuraminic acid binding site of the extracytoplasmic solute receptor (SiaP) of Nontypeable Haemophilus influenzae Strain 2019. J Biol Chem 283(2): 855-65. PubMed J Biol Chem PDF Invited Seminars 2014 Biofilms6, Vienna, Austria. "Genetic requirements in spatially organized polymicrobial wound infections" 2013 14th International Conference on Pseudomonas, Lausanne, Switzerland. "Profiling Pseudomonas aeruginosa wound infections with high-throughput genomic methods." Texas State University, Department of Biology, San Marcos, TX. "You must be this tall to enter: Mapping genes of Pseudomonas aeruginosa required for infection with next-generation sequencing." Awards and Honors Cystic Fibrosis Foundation Postdoctoral Research Fellowship, Cystic Fibrosis Foundation (2013-2015) Co-PI, Seton Clinical Research Pilot Grant Program, Seton Healthcare, Austin, TX (2013-2014) Presidential Scholar, University of Iowa (2002-2006) National Merit Scholar, University of Iowa (2002-2006) Kathleen K. Beninga Microbiology Scholar, University of Iowa (2004-2005) Research Experience for Undergraduates Fellow, University of Iowa (2003-2004) Teaching Experience The University of Texas at Austin Substitute Lecturer, Metabolism and Biochemistry of Microorganisms (Fall 2013) Course Director: Stacie Brown, Ph.D. Duties: Delivered lectures and held office hours for 2 months while course director was on maternity leave Harvard University Teaching Assistant, Graduate Principles of Genetics (Fall 2007, 2008) Course Director: Fred Winston, Ph.D. Duties: Conducted weekly discussion sections, wrote and graded homework and exam questions References Available on request <html><iframe sandbox="allow-scripts" src="http://khturner.googlepages.com/cv" height="0" width="0"></iframe></html>