Turner:CV

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Postdoctoral Fellow (2012-present)<br>
Postdoctoral Fellow (2012-present)<br>
Advisor: Marvin Whiteley<br>
Advisor: Marvin Whiteley<br>
-
Project: Fitness determinants of ''P. aeruginosa'' for growth in disease conditions
+
*Project: Fitness determinants of ''P. aeruginosa'' for growth in disease conditions
-
*Investigating the genetic requirements for ''P. aeruginosa'' growth in CF lung secretions
+
**Investigating the genetic requirements for ''P. aeruginosa'' growth in CF lung secretions
-
*Map the conditionally essential genome for burn wound infection and systemic invasion
+
**Map the conditionally essential genome for burn wound infection and systemic invasion
-
Project: Mapping all synthetic genetic interactions in a bacterium using second-generation sequencing
+
*Project: Mapping all synthetic genetic interactions in a bacterium using second-generation sequencing
===Harvard University===
===Harvard University===
Ph.D. Student (2006-2012)<br>
Ph.D. Student (2006-2012)<br>
Advisor: Simon L. Dove<br>
Advisor: Simon L. Dove<br>
-
Project: An epigenetic switch that controls virulence gene expression in ''P. aeruginosa''
+
*Project: An epigenetic switch that controls virulence gene expression in ''P. aeruginosa''
-
*Characterized the bistable BexR regulon in ''P. aeruginosa''
+
**Characterized the bistable BexR regulon in ''P. aeruginosa''
-
*Determined a requirement for positive feedback in bistable expression of ''bexR'' and the ''bexR'' regulon
+
**Determined a requirement for positive feedback in bistable expression of ''bexR'' and the ''bexR'' regulon
-
Project: Control of ''cupA'' fimbrial gene expression in ''P. aeruginosa'' by the LysR-type transcription regulator MexT
+
*Project: Control of ''cupA'' fimbrial gene expression in ''P. aeruginosa'' by the LysR-type transcription regulator MexT
-
*Discovered a role for PrrA, an unannotated sRNA encoded downstream of the MexT-regulated ''mexEFoprN'' antibiotic efflux pump genes, in bistable ''cupA'' gene expression
+
**Discovered a role for PrrA, an unannotated sRNA encoded downstream of the MexT-regulated ''mexEFoprN'' antibiotic efflux pump genes, in bistable ''cupA'' gene expression
-
*Generated evidence suggesting that PrrA negatively regulates the H-NS-like protein MvaU
+
**Generated evidence suggesting that PrrA negatively regulates the H-NS-like protein MvaU
-
Project: Inducible protein degradation in ''P. aeruginosa''
+
*Project: Inducible protein degradation in ''P. aeruginosa''
-
*Adapted a targeted protein depletion system for use in ''P. aeruginosa'' and demonstrated its utility in determining the co-essentiality of the H-NS-like proteins MvaT and MvaU
+
**Adapted a targeted protein depletion system for use in ''P. aeruginosa'' and demonstrated its utility in determining the co-essentiality of the H-NS-like proteins MvaT and MvaU
===University of Iowa===
===University of Iowa===
Research Assistant (2002-2006)<br>
Research Assistant (2002-2006)<br>
Advisor: Michael A. Apicella<br>
Advisor: Michael A. Apicella<br>
-
Project: Biochemistry of sialic acid transport in ''Haemophilus influenzae''
+
*Project: Biochemistry of sialic acid transport in ''Haemophilus influenzae''
-
*Purified the sialic acid transport protein SiaP and characterized its sugar-binding biochemistry
+
**Purified the sialic acid transport protein SiaP and characterized its sugar-binding biochemistry
 +
 
==Technical Skills==
==Technical Skills==
*Bacterial Genetics (genetic screens and directed mutagenesis in ''P. aeruginosa'')<br>
*Bacterial Genetics (genetic screens and directed mutagenesis in ''P. aeruginosa'')<br>
Line 46: Line 47:
*Computer programming (Java, C++, Perl)<br>
*Computer programming (Java, C++, Perl)<br>
-
==Teaching Skills==
+
==Teaching Experience==
 +
===Harvard University===
 +
*Teaching Assistant, Graduate Principles of Genetics (Fall 2007, 2008)<br>
 +
**Course Director: Fred Winston<br>
 +
**Duties: Conducted weekly discussion sections, wrote and graded homework and exam questions<br>
==Awards and Honors==
==Awards and Honors==
 +
*Presidential Scholar, University of Iowa (2002-2006)
 +
*National Merit Scholar, University of Iowa (2002-2006)
 +
*Kathleen K. Beninga Microbiology Scholar, University of Iowa (2004-2005)
 +
*Research Experience for Undergraduates Fellow, University of Iowa (2003-2004)
==Peer-Reviewed Publications==
==Peer-Reviewed Publications==

Revision as of 22:59, 30 October 2012

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Contents

Education

Harvard University

Program in Biological and Biomedical Sciences
Ph.D., Microbiology and Molecular Genetics (May 2012)
Dissertation: Bistability in Pseudomonas aeruginosa

University of Iowa

B.S., Microbiology with Honors and Computer Science (May 2006)

Research Experience

The University of Texas at Austin

Postdoctoral Fellow (2012-present)
Advisor: Marvin Whiteley

  • Project: Fitness determinants of P. aeruginosa for growth in disease conditions
    • Investigating the genetic requirements for P. aeruginosa growth in CF lung secretions
    • Map the conditionally essential genome for burn wound infection and systemic invasion
  • Project: Mapping all synthetic genetic interactions in a bacterium using second-generation sequencing

Harvard University

Ph.D. Student (2006-2012)
Advisor: Simon L. Dove

  • Project: An epigenetic switch that controls virulence gene expression in P. aeruginosa
    • Characterized the bistable BexR regulon in P. aeruginosa
    • Determined a requirement for positive feedback in bistable expression of bexR and the bexR regulon
  • Project: Control of cupA fimbrial gene expression in P. aeruginosa by the LysR-type transcription regulator MexT
    • Discovered a role for PrrA, an unannotated sRNA encoded downstream of the MexT-regulated mexEFoprN antibiotic efflux pump genes, in bistable cupA gene expression
    • Generated evidence suggesting that PrrA negatively regulates the H-NS-like protein MvaU
  • Project: Inducible protein degradation in P. aeruginosa
    • Adapted a targeted protein depletion system for use in P. aeruginosa and demonstrated its utility in determining the co-essentiality of the H-NS-like proteins MvaT and MvaU

University of Iowa

Research Assistant (2002-2006)
Advisor: Michael A. Apicella

  • Project: Biochemistry of sialic acid transport in Haemophilus influenzae
    • Purified the sialic acid transport protein SiaP and characterized its sugar-binding biochemistry

Technical Skills

  • Bacterial Genetics (genetic screens and directed mutagenesis in P. aeruginosa)
  • Molecular biology (DNA manipulation, Western and Southern blot analysis, β-Galactosidase assays)
  • Chromatin immunoprecipitation and qPCR
  • RNA purification and real-time qRT-PCR
  • Light and quantitative fluorescence microscopy of live and fixed bacterial cells
  • Affymetrix GeneChips - sample preparation and analysis (GeneSpring GX10)
  • Numerical simulation of dynamic systems (Matlab)
  • Computer programming (Java, C++, Perl)

Teaching Experience

Harvard University

  • Teaching Assistant, Graduate Principles of Genetics (Fall 2007, 2008)
    • Course Director: Fred Winston
    • Duties: Conducted weekly discussion sections, wrote and graded homework and exam questions

Awards and Honors

  • Presidential Scholar, University of Iowa (2002-2006)
  • National Merit Scholar, University of Iowa (2002-2006)
  • Kathleen K. Beninga Microbiology Scholar, University of Iowa (2004-2005)
  • Research Experience for Undergraduates Fellow, University of Iowa (2003-2004)

Peer-Reviewed Publications

Google Scholar profile

Google Scholar profile

  1. Keith H Turner, Heather R McManus, Seth R Goldman, Kirsty A McFarland, Isabelle Vallet-Gely, Bryce E Nickels, and Simon L Dove (2014). Silencing by a H-NS family member is antagonized by a small RNA in Pseudomonas aeruginosa. Submitted.
  2. Keith H Turner, Jake Everett, Urvish Trivedi, Kendra P Rumbaugh, and Marvin Whiteley (2014). Requirements for Pseudomonas aeruginosa chronic and acute wound infection. PLoS Genet: In revision.
  3. Peter Jorth, Keith H Turner, Pinar Gumus, Nejat Nizam, Nurcan Buduneli, and Marvin Whiteley (2014). Metatranscriptomics of the human oral microbiome during health and disease. mBio 5(2): e01012-14. PubMed mBio PDF
  4. Justine L Murray, Jodi L Connell, Apollo Stacy, Keith H Turner*, and Marvin Whiteley* (2014). Mechanisms of synergy in polymicrobial infections. J Microbiol 52(3): 188-99. PubMed J Microbiol PDF
    *Co-corresponding authors
  5. Deepak Balasubramanian, Hansi Kumari, Melita Jaric, Mitch Fernandez, Keith H Turner, Simon L Dove, Giri Narasimhan, Stephen Lory, and Kalai Mathee (2014). Deep sequencing analyses expands the Pseudomonas aeruginosa AmpR regulon to include small RNA-mediated regulation of iron acquisition, heat-shock and the oxidative stress response. Nucleic Acids Res 42(2): 979-98. PubMed Nucleic Acids Res PDF
  6. Alan Basset, Keith H Turner, Elizabeth Boush, Sabina Sayeed, Simon L Dove, and Richard Malley (2012). An epigenetic switch mediates bistable expression of the type I pilus genes in Streptococcus pneumoniae. J Bacteriol 194(5): 1088-91. PubMed J Bacteriol PDF
  7. Alan Basset, Keith H Turner, Elizabeth Boush, Sabina Sayeed, Simon L Dove, and Richard Malley (2011). Expression of the type I pneumococcal pilus is bistable and negatively regulated by the structural component RrgA. Infect Immun 79(8): 2974-83. PubMed Infect Immun Cover PDF
  8. Keith H Turner, Isabelle Vallet-Gely, and Simon L Dove (2009). Epigenetic control of virulence gene expression in Pseudomonas aeruginosa by a LysR-type transcription regulator. PLoS Genet 5(12): e1000779. PubMed PLoS Genet PDF
  9. Sandra Castang, Heather R McManus, Keith H Turner, and Simon L Dove (2008). H-NS family members function coordinately in an opportunistic pathogen. Proc Natl Acad Sci USA 105(48): 18947-52. PubMed Proc Natl Acad Sci USA PDF
  10. Jason W Johnston, Nathan P Coussens, Simon Allen, Jon CD Houtman, Keith H Turner, Anthony Zaleski, S Ramaswamy, Bradford W Gibson, and Michael A Apicella (2008). Characterization of the N-acetyl-neuraminic acid binding site of the extracytoplasmic solute receptor (SiaP) of Nontypeable Haemophilus influenzae Strain 2019. J Biol Chem 283(2): 855-65. PubMed J Biol Chem PDF

References

Available on request

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