Turner:Solutions/GAMBITMgRxn

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: ==GAMBIT 2x Reaction Buffer + Mg - 10 mL== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Component''' | align="center" style="background:#f0f0f0;"|'''Final []''' | align="ce...)
Current revision (09:02, 31 October 2012) (view source)
 
Line 21: Line 21:
|  
|  
|-
|-
-
| pH to||7.9||with||x||(approx)||x||mL
+
| pH to||7.9||with||_||(approx)||_||mL
|-
|-
| sterilize by||f.s.||||||||||
| sterilize by||f.s.||||||||||

Current revision

GAMBIT 2x Reaction Buffer + Mg - 10 mL

Component Final [] Stock [] Units mL stock ' '
Glycerol2050%4
HEPES pH 7.90.051M0.5
DTT0.0040.1M0.4
NaCl0.25M0.4
MgCl20.011M0.1
pH to7.9with_(approx)_mL
sterilize byf.s.
store at4C
Note: use 100x BSA from NEB as 40x stock for GAMBIT reaction
Personal tools