Turner:Solutions/GAMBITTWB: Difference between revisions
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(New page: ==GAMBIT TWB - 50 mL== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Component''' | align="center" style="background:#f0f0f0;"|'''Final []''' | align="center" style="backgro...) |
(New page: ==GAMBIT TWB - 50 mL== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Component''' | align="center" style="background:#f0f0f0;"|'''Final []''' | align="center" style="backgro...) |
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Latest revision as of 06:58, 31 October 2012
GAMBIT TWB - 50 mL
Component | Final [] | Stock [] | Units | mL stock | ' | ' |
Tris-HCl pH 7.4 | 0.02 | 1 | M | 1 | ||
NaCl | 0.2 | 5 | M | 2 | ||
EDTA pH 8 | 0.001 | 0.5 | M | 0.1 | ||
DTT | 0.002 | 0.1 | M | 1 | ||
Glycerol | 10 | 50 | % | 10 | ||
pH to | 7.4 | with | 5N HCl | (approx) | 2 | drops |
sterilize by | f.s. | |||||
store at | 4C | |||||
Note: add 1 tablet cOmplete mini, EDTA-free to 10 mL buffer before use |