Tween 20
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Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses. | Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses. | ||
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| + | == Storage/Stability == | ||
| + | Based on the SIGMA Tween20 data sheet [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/p5927pis.pdf] | ||
| + | * aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate | ||
| + | * autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. | ||
| + | * TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures | ||
| + | * polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid | ||
| + | * polysorbates may reduce the activity of many preservatives. No plastic incompatabilities have been observed. | ||
== See also == | == See also == | ||
Revision as of 13:43, 14 October 2008
Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.
Storage/Stability
Based on the SIGMA Tween20 data sheet [1]
- aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate
- autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions.
- TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures
- polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
- polysorbates may reduce the activity of many preservatives. No plastic incompatabilities have been observed.
