Tween 20

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Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.
Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.
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== Storage/Stability ==
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Based on the SIGMA Tween20 data sheet [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/p5927pis.pdf]
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* aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate
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* autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions.
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* TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures
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* polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
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* polysorbates may reduce the activity of many preservatives. No plastic incompatabilities have been observed.
== See also ==
== See also ==

Revision as of 13:43, 14 October 2008

Structure of Tween20, aka Polyoxyethylene (20) sorbitan monolaurate
Structure of Tween20, aka Polyoxyethylene (20) sorbitan monolaurate

Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.

Storage/Stability

Based on the SIGMA Tween20 data sheet [1]

  • aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate
  • autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions.
  • TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures
  • polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
  • polysorbates may reduce the activity of many preservatives. No plastic incompatabilities have been observed.

See also

  • TBST - tris-buffered saline with Tween
  • PBS-T - phosphate-buffered saline with Tween

External links

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