Tween 20: Difference between revisions
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[[Image:250px-Polysorbate 20.png|thumb|250px|Structure of Tween20, aka Polyoxyethylene (20) sorbitan monolaurate]] | |||
'''Tween''' (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses. | |||
== Storage/Stability == | |||
* 2 years shelf life according to this chemicals manufacturer [http://www.jyhychem.com/pages/pro201_en.htm] | |||
* 2 years for 20x Tris-buffered saline/Tween20 according to this data sheet [http://search.funakoshi.co.jp/data/datasheet/LVC/TA-XXX-TT.pdf] | |||
Based on the SIGMA Tween20 data sheet [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/p5927pis.pdf] | |||
* aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate | |||
* autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. | |||
* TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures | |||
* polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid | |||
* polysorbates may reduce the activity of many preservatives | |||
* no plastic incompatabilities have been observed | |||
== Comments == | |||
* "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a <u>new bottle of tween-20 (BDH) there are big reductions in the background</u>. OK, so this may have been expected, but not to the extent that I have noticed. Both of the 'old' reagents had probably been opened less than 6 months ago." [http://www.bio.net/bionet/mm/diagnost/1997-May/001119.html] | |||
== See also == | |||
* [[TBST]] - tris-buffered saline with Tween | |||
* [[PBS-T]] - phosphate-buffered saline with Tween | |||
* [[Critical micelle concentration (CMC)]] | |||
== External links == | |||
* [http://en.wikipedia.org/wiki/Tween_20 Tween 20] on Wikipedia | |||
* [http://en.wikipedia.org/wiki/Tween_80 Tween 80] on Wikipedia | |||
[[Category:Material]] | [[Category:Material]] | ||
[[Category:Detergents]] | [[Category:Detergents]] |
Latest revision as of 13:05, 29 July 2009
Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.
Storage/Stability
- 2 years shelf life according to this chemicals manufacturer [1]
- 2 years for 20x Tris-buffered saline/Tween20 according to this data sheet [2]
Based on the SIGMA Tween20 data sheet [3]
- aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate
- autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions.
- TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures
- polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
- polysorbates may reduce the activity of many preservatives
- no plastic incompatabilities have been observed
Comments
- "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a new bottle of tween-20 (BDH) there are big reductions in the background. OK, so this may have been expected, but not to the extent that I have noticed. Both of the 'old' reagents had probably been opened less than 6 months ago." [4]
See also
- TBST - tris-buffered saline with Tween
- PBS-T - phosphate-buffered saline with Tween
- Critical micelle concentration (CMC)