Tween 20 - Revision history

Jakob Suckale: /* See also */

2009-07-29T20:05:21Z

2009-07-28T13:23:50Z

Tween moved to Tween 20 over redirect: more precise description

←Older revision

Revision as of 13:23, 28 July 2009

2008-10-14T18:41:07Z

←Older revision		Revision as of 18:41, 14 October 2008
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	[[Image:250px-Polysorbate 20.png\|thumb\|250px\|Structure of Tween20, aka Polyoxyethylene (20) sorbitan monolaurate]]		[[Image:250px-Polysorbate 20.png\|thumb\|250px\|Structure of Tween20, aka Polyoxyethylene (20) sorbitan monolaurate]]

-	Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.	+	'''Tween''' (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.

	== Storage/Stability ==		== Storage/Stability ==

2008-10-14T18:02:09Z

Storage/Stability

←Older revision		Revision as of 18:02, 14 October 2008
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	* TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures		* TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures
	* polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid		* polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
-	* polysorbates may reduce the activity of many preservatives~~. No~~ plastic incompatabilities have been observed.	+	* polysorbates may reduce the activity of many preservatives
		+	* no plastic incompatabilities have been observed

	== Comments ==		== Comments ==

2008-10-14T17:57:12Z

Storage/Stability

←Older revision		Revision as of 17:57, 14 October 2008
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	== Storage/Stability ==		== Storage/Stability ==
		+	* 2 years shelf life according to this chemicals manufacturer [http://www.jyhychem.com/pages/pro201_en.htm]
		+	* 2 years for 20x Tris-buffered saline/Tween20 according to this data sheet [http://search.funakoshi.co.jp/data/datasheet/LVC/TA-XXX-TT.pdf]
		+
	Based on the SIGMA Tween20 data sheet [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/p5927pis.pdf]		Based on the SIGMA Tween20 data sheet [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/p5927pis.pdf]
	* aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate		* aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate

2008-10-14T17:48:39Z

Comments

←Older revision		Revision as of 17:48, 14 October 2008
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	== Comments ==		== Comments ==
-	* "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a <u>new bottle of tween-20 (BDH) there are big reductions in the background</u>." [http://www.bio.net/bionet/mm/diagnost/1997-May/001119.html]	+	* "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a <u>new bottle of tween-20 (BDH) there are big reductions in the background</u>. OK, so this may have been expected, but not to the extent that I have noticed. Both of the 'old' reagents had probably been opened less than 6 months ago." [http://www.bio.net/bionet/mm/diagnost/1997-May/001119.html]

	== See also ==		== See also ==

2008-10-14T17:48:00Z

comments

←Older revision		Revision as of 17:48, 14 October 2008
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	* polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid		* polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
	* polysorbates may reduce the activity of many preservatives. No plastic incompatabilities have been observed.		* polysorbates may reduce the activity of many preservatives. No plastic incompatabilities have been observed.
		+
		+	== Comments ==
		+	* "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a <u>new bottle of tween-20 (BDH) there are big reductions in the background</u>." [http://www.bio.net/bionet/mm/diagnost/1997-May/001119.html]

	== See also ==		== See also ==

2008-10-14T17:43:36Z

storage/stability

←Older revision		Revision as of 17:43, 14 October 2008
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	Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.		Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.
		+
		+	== Storage/Stability ==
		+	Based on the SIGMA Tween20 data sheet [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/p5927pis.pdf]
		+	* aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate
		+	* autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions.
		+	* TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures
		+	* polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
		+	* polysorbates may reduce the activity of many preservatives. No plastic incompatabilities have been observed.

	== See also ==		== See also ==

2008-10-14T17:35:41Z

illustration

←Older revision		Revision as of 17:35, 14 October 2008
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		+	[[Image:250px-Polysorbate 20.png\|thumb\|250px\|Structure of Tween20, aka Polyoxyethylene (20) sorbitan monolaurate]]
		+
	Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.		Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.

2008-10-14T17:30:35Z

external links

←Older revision		Revision as of 17:30, 14 October 2008
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	Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.		Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.
-

	== See also ==		== See also ==
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	* [[PBS-T]] - phosphate-buffered saline with Tween		* [[PBS-T]] - phosphate-buffered saline with Tween

		+	== External links ==
		+	* [http://en.wikipedia.org/wiki/Tween_20 Tween 20] on Wikipedia
		+	* [http://en.wikipedia.org/wiki/Tween_80 Tween 80] on Wikipedia

	[[Category:Material]]		[[Category:Material]]
	[[Category:Detergents]]		[[Category:Detergents]]

←Older revision		Revision as of 20:05, 29 July 2009
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	* [[TBST]] - tris-buffered saline with Tween		* [[TBST]] - tris-buffered saline with Tween
	* [[PBS-T]] - phosphate-buffered saline with Tween		* [[PBS-T]] - phosphate-buffered saline with Tween
		+	* [[Critical micelle concentration (CMC)]]

	== External links ==		== External links ==