UA Biophysics:Membrane Dynamics

MEMBRANE BIOPHYSICS PI: None Summary We have developed a multi-approach strategy involving spectroscopy and microscopy techniques to explore how the physical properties of cell membranes influence a variety of physiological mechanisms that range from cell signaling to membrane mechanical properties. Techniques • Fluorescence spectroscopy - ISS PC1 Spectrofluorometer • Fluorescence microscopy - Zeiss Axiovert 40 CFL, Nikon • Atomic force microscopy - MFP-3D-BIO AFM Asylum Research Current projects • Nanomechanical properties of supported lipid bilayers in presence of the antimicrobial peptide Magainin-H2 - PhD student: Nathaly Marín-Medina (nmarin at uniandes.edu.co) • Master student: Rudy Marcela Méndez Ancient projects • Influence of lipid domain formation and membrane structure on PLA2 activity. This hydrolytic enzyme is highly sensitive to the physical properties of the bilayer membrane. We explore how the modulation of membrane lateral organization can act as a regulator of the enzyme. • Physical properties of bacterial membranes and their influence on the susceptibility of bacterial populations towards antibacterial agents. • Modeling the motion of giant uniilamellar vesicles interacting with surfaces (an experimental and computational project in collaboration with Dr. Andres Gonzalez in Mechanical Engineering) • Modulating membrane fusion through the activity of hydrolytic enzymes and membrane active peptides. • Modeling the effects of rhodopsin organization in regulating the dynamics of single photon response.