UA Biophysics:Microscopy: Difference between revisions
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We use commercial microscopes (in our lab, and in the microscopy center) for some our experiments and we also build our own microscopes. Our experience is mainly in fluorescence microscopy, Force Spectroscopy, Atomic Force Microscopy and Light Sheet Fluorescence Microscopy ([https://en.wikipedia.org/wiki/Light_sheet_fluorescence_microscopy LSFM]). | We use commercial microscopes (in our lab, and in the microscopy center) for some our experiments and we also build our own microscopes. Our experience is mainly in fluorescence microscopy, Force Spectroscopy, Atomic Force Microscopy and Light Sheet Fluorescence Microscopy ([https://en.wikipedia.org/wiki/Light_sheet_fluorescence_microscopy LSFM]). | ||
• We built a type of LSFM, a Single Plane Illumination Microscope (SPIM) in collaboration with [http://damcb.com/ Dr. Guillaume Gay]. This microscope gets us 3D images of microscopic objects such as sclerites of coral (the skeleton of coral) in order to assess the impact of ocean acidification on the growth of corals in collaboration with [http://biommar.uniandes.edu.co/ Biommar]. This type of microscope is particularly interesting for minimizing photobleaching in development studies, generating 3D sections of relatively large specimens in biology as well as tracking single particles with low background. We have are also studying zebrafish in collaboration with Students in physics and engineering are encouraged to apply. | • We built a type of LSFM, a Single Plane Illumination Microscope (SPIM) in collaboration with [http://damcb.com/ Dr. Guillaume Gay]. This microscope gets us 3D images of microscopic objects such as sclerites of coral (the skeleton of coral) in order to assess the impact of ocean acidification on the growth of corals in collaboration with [http://biommar.uniandes.edu.co/ Biommar]. This type of microscope is particularly interesting for minimizing photobleaching in development studies, generating 3D sections of relatively large specimens in biology as well as tracking single particles with low background. We have are also studying zebrafish in collaboration with [http://neurolab.uniandes.edu.co/index.html Laboratorio de Neurociencia y Ritmos Circadianos]. Students in physics, biology, medicine and engineering are encouraged to apply. | ||
• We built an Atomic Force Microscope to do single molecule studies of molecules with support of the Fernandez lab at Columbia University. We will be doing force spectroscopy of biologically-relevant molecules to understand their mechanics. Students in physics with interests in biophysics or instrumentation development are encouraged to apply. In the near future we expect to study the mechanical properties of biological membranes, proteins in neurons as well as cell mechanics in neurons. | • We built an Atomic Force Microscope to do single molecule studies of molecules with support of the Fernandez lab at Columbia University. We will be doing force spectroscopy of biologically-relevant molecules to understand their mechanics. Students in physics with interests in biophysics or instrumentation development are encouraged to apply. In the near future we expect to study the mechanical properties of biological membranes, proteins in neurons as well as cell mechanics in neurons. | ||
Revision as of 09:42, 30 August 2015
Microscopy
We have experience in several types of microscopy with two goals in mind: 1-To do our own research and 2-Help colleagues in their microscopy needs. We use commercial microscopes (in our lab, and in the microscopy center) for some our experiments and we also build our own microscopes. Our experience is mainly in fluorescence microscopy, Force Spectroscopy, Atomic Force Microscopy and Light Sheet Fluorescence Microscopy (LSFM).
• We built a type of LSFM, a Single Plane Illumination Microscope (SPIM) in collaboration with Dr. Guillaume Gay. This microscope gets us 3D images of microscopic objects such as sclerites of coral (the skeleton of coral) in order to assess the impact of ocean acidification on the growth of corals in collaboration with Biommar. This type of microscope is particularly interesting for minimizing photobleaching in development studies, generating 3D sections of relatively large specimens in biology as well as tracking single particles with low background. We have are also studying zebrafish in collaboration with Laboratorio de Neurociencia y Ritmos Circadianos. Students in physics, biology, medicine and engineering are encouraged to apply.
• We built an Atomic Force Microscope to do single molecule studies of molecules with support of the Fernandez lab at Columbia University. We will be doing force spectroscopy of biologically-relevant molecules to understand their mechanics. Students in physics with interests in biophysics or instrumentation development are encouraged to apply. In the near future we expect to study the mechanical properties of biological membranes, proteins in neurons as well as cell mechanics in neurons.
