UA Biophysics:Protocols: Difference between revisions
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LB protocol | LB protocol | ||
Mix dry ingredients and add distilled water up to less than 1 Liter | |||
After dissolved, add agar | |||
Pour into 2 L flask (or greater) | |||
Autoclave (liquid cycle) | |||
250°F, 22psi, 30 minutes | |||
Cool media down to 60-55C (uncomfortable but not painful) | |||
Add appropriate antibiotic | |||
Pour the solution on sterile (???) plates | |||
Let solidify about 1 h, wrap, label (name, date, additive) | |||
keep at 4°C | |||
Revision as of 14:18, 9 July 2015
LB protocol
Mix dry ingredients and add distilled water up to less than 1 Liter After dissolved, add agar Pour into 2 L flask (or greater) Autoclave (liquid cycle) 250°F, 22psi, 30 minutes Cool media down to 60-55C (uncomfortable but not painful) Add appropriate antibiotic Pour the solution on sterile (???) plates Let solidify about 1 h, wrap, label (name, date, additive) keep at 4°C
