UA Biophysics:Protocols:M9 Salts (5x) 1L: Difference between revisions
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M9 Salts (5X) 1L | M9 Salts (5X) 1L | ||
*64 g Na<sub>2</sub>HPO<sub>4</sub>7H<sub>2</sub>O Sodium Phosphate Dibasic Heptahydrate | *64 g Na<sub>2</sub>HPO<sub>4</sub>7H<sub>2</sub>O Sodium Phosphate Dibasic Heptahydrate '''OR''' 33.9 g Na<sub>2</sub>HPO<sub>4</sub> Sodium Phosphate Dibasic Anhydrous | ||
*15 g KH<sub>2</sub>PO<sub>4</sub> Potassium Phosphate | *15 g KH<sub>2</sub>PO<sub>4</sub> Potassium Phosphate | ||
*2.5 g Na Cl Sodium Chloride | *2.5 g Na Cl Sodium Chloride | ||
Revision as of 06:14, 10 July 2015
M9 Salts (5X) 1L
- 64 g Na2HPO47H2O Sodium Phosphate Dibasic Heptahydrate OR 33.9 g Na2HPO4 Sodium Phosphate Dibasic Anhydrous
- 15 g KH2PO4 Potassium Phosphate
- 2.5 g Na Cl Sodium Chloride
- 5 g NH4Cl Ammonium Chloride
Fill the bottle with MilliQ until 1 L pH should be around 7.4. BE CAREFUL AUTOCLAVE
