UA Biophysics:Protocols:Stocks: Difference between revisions
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(New page: Method #Bacteria taken from either Keio tubes, or remaining stock and streaked onto antibiotic LB agar plates #Grow plates overnight in hot room, collect early next day to get small colo...) |
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Revision as of 12:58, 23 July 2015
Method
- Bacteria taken from either Keio tubes, or remaining stock and streaked onto antibiotic LB agar plates
- Grow plates overnight in hot room, collect early next day to get small colonies
Select small single colony and add to 10ml LB (with antibiotics if resistance is in the strain) portioned into conical flask Place flask in hot room on shaker for overnight growth Collect flask early next day
LB:Glycerol(50%) 3ml:2ml ratio Portion grown bacteria into eppendorfs (130ul each) Freeze bacteria in liquid nitrogen Store in -80C freezer 1:1000 dilution of 100mg/ml amp 50mg/ml kan
