UCBigem09-Protocols: Difference between revisions
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<li | <li>[[BerkiGEMwet2009 | Home]]</li> | ||
<li>[[UCBigem09-Protocols | Protocols]]</li> | <li id="current">[[UCBigem09-Protocols | Protocols]]</li> | ||
<li>[[Notebooks | Notebooks]]</li> | <li>[[Notebooks | Notebooks]]</li> | ||
<li>[[Construction Files | ConstructionFiles ]] </li> | <li>[[Construction Files | ConstructionFiles ]] </li> | ||
Revision as of 10:23, 18 June 2009
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University of California Berkeley iGem 2009 Wetlab Team |
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All these protocols in one big list
Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification
Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)
Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Machery-Nagel Miniprep purification of DNA
Other
LR Gateway Transfers
What do I do with my sequencing data?
