UCBigem09-Protocols: Difference between revisions
John Yi Wang (talk | contribs) |
|||
| Line 56: | Line 56: | ||
[[Template:Colony PCR | Colony PCR]]<br> | [[Template:Colony PCR | Colony PCR]]<br> | ||
=== | ===Binding/Display assay protocols=== | ||
[[Template:SBB- Streptavidin Display Assay Microscopy | Streptavidin Display Assay - Microscopy]]<br> | [[Template:SBB- Streptavidin Display Assay Microscopy | Streptavidin Display Assay - Microscopy]]<br> | ||
[[Template:SBB- Streptavidin TECAN Assay | Streptavidin Display Assay - TECAN]]<br> | [[Template:SBB- Streptavidin TECAN Assay | Streptavidin Display Assay - TECAN]]<br> | ||
[[Template:SBB- Streptavidin ELESA | Streptavidin Display Assay - ELESA]]<br> | [[Template:SBB- Streptavidin ELESA | Streptavidin Display Assay - ELESA]]<br> | ||
Revision as of 15:25, 26 June 2009
|
University of California Berkeley iGem 2009 Wetlab Team |
|---|
All these protocols in one big list
Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
Template:SBB-Protocols_PCR4 ! PCA gene synthesis
Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification
Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)
EcoRI/BamHI Digest of vector plasmid
Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Machery-Nagel Miniprep purification of DNA
Other
LR Gateway Transfers
What do I do with my sequencing data?
Reaction Guides
SOEing with ProTips
Colony PCR
Binding/Display assay protocols
Streptavidin Display Assay - Microscopy
Streptavidin Display Assay - TECAN
Streptavidin Display Assay - ELESA
