UCBigem09-Protocols: Difference between revisions
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[[Template:SBB- Streptavidin Pellet Assay | Streptavidin Display Assay - Photographing Pellets + TECAN]]<br> | [[Template:SBB- Streptavidin Pellet Assay | Streptavidin Display Assay - Photographing Pellets + TECAN]]<br> | ||
[[Template:SBB- Surface-binding Assay | Surface-binding Assay]]<br> | [[Template:SBB- Surface-binding Assay | Surface-binding Assay]]<br> | ||
[[Template:SBB- Growth Assay | Growth Assay]]<br> | [[Template:SBB- Growth Rate Assay | Growth Rate Assay]]<br> | ||
===Assembly protocols=== | ===Assembly protocols=== | ||
[[Template:Assemby Protocol | Assembly Protocol]]<br> | [[Template:Assemby Protocol | Assembly Protocol]]<br> | ||
Revision as of 11:29, 22 July 2009
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University of California Berkeley iGem 2009 Wetlab Team |
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All these protocols in one big list
Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
PCA gene synthesis
Colony PCR
Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification
Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)
EcoRI/BamHI Digest of vector plasmid
Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Machery-Nagel Miniprep purification of DNA
Other
LR Gateway Transfers
What do I do with my sequencing data?
In Vivo Gateway
Reaction Guides
SOEing with ProTips
Colony PCR
Binding/Display assay protocols
Streptavidin Display Assay - Microscopy - do longer in development
Streptavidin Display Assay - TECAN
Streptavidin Display Assay - ELESA - no longer in development
Streptavidin Display Assay - Photographing Pellets + TECAN
Surface-binding Assay
Growth Rate Assay
