UCBigem09-Protocols: Difference between revisions
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University of California Berkeley iGem 2009 Wetlab Team<br> | |||
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<li>[[BerkiGEMwet2009 | Home]]</li> | |||
<li id="current">[[UCBigem09-Protocols | Protocols]]</li> | |||
<li>[[Data| Data]]</li> | |||
<li>[[Notebooks | Notebooks]]</li> | |||
<li>[[Construction Files | ConstructionFiles ]] </li> | |||
<li>[[References_and_Papers | References and Papers]]</li> | |||
<li>[[Parts Database|Parts Database]] | |||
<li>[[UCBigem09-groupmtg|Group Mtg Presentations]] | |||
<li>[[About_Us | About Us]]</li> | |||
</ul> | |||
</div> | |||
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[[SBB-Protocols_all | All these protocols in one big list]]<br> | [[SBB-Protocols_all | All these protocols in one big list]]<br> | ||
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[[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br> | [[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br> | ||
[[Template:SBB-Protocols_PCR3 | SOEing PCR]]<br> | [[Template:SBB-Protocols_PCR3 | SOEing PCR]]<br> | ||
[[Template:SBB-Protocols_PCR4 | PCA gene synthesis]]<br> | |||
[[Template:SBB-Protocols_PCR5 | Colony PCR]]<br> | |||
===Cleanup Reactions=== | ===Cleanup Reactions=== | ||
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[[Template:SBB-Protocols_Enz5 | Ligation of EIPCR digests]]<br> | [[Template:SBB-Protocols_Enz5 | Ligation of EIPCR digests]]<br> | ||
[[Template:SBB-Protocols_Enz6 | Analytical digests (Mapping)]]<br> | [[Template:SBB-Protocols_Enz6 | Analytical digests (Mapping)]]<br> | ||
[[Template:SBB-Protocols_Enz7 | EcoRI/BamHI Digest of vector plasmid]]<br> | |||
===Bacteria Manipulations=== | ===Bacteria Manipulations=== | ||
[[Template:SBB-Protocols_Micro1 | Transformation by heat-shock]]<br> | [[Template:SBB-Protocols_Micro1 | Transformation by heat-shock]]<br> | ||
[[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br> | [[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br> | ||
===Other=== | ===Other=== | ||
[[Template:SBB-Protocols_LRGtw | LR Gateway Transfers]]<br> | [[Template:SBB-Protocols_LRGtw | LR Gateway Transfers]]<br> | ||
[[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br> | [[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br> | ||
[[Template:SBB-Protocols_inVivoGateway | In Vivo Gateway]]<br> | |||
[[Template:SBB-Protocols_EcoBamTransfer | EcoRI/BamHI Transfer]]<br> | |||
===Reaction Guides=== | |||
[[Template:SBB-SOEing | SOEing with ProTips]]<br> | |||
[[Template:Colony PCR | Colony PCR]]<br> | |||
=== | ===Binding/Display assay protocols=== | ||
[[Template:SBB- | [[Template:SBB- Streptavidin Display Assay Microscopy | Streptavidin Display Assay - Microscopy - do longer in development]]<br> | ||
[[Template:SBB- Streptavidin TECAN Assay | Streptavidin Display Assay - TECAN]]<br> | |||
[[Template:SBB- Streptavidin ELESA | Streptavidin Display Assay - ELESA - no longer in development]]<br> | |||
[[Template:SBB- Streptavidin Pellet Assay | Streptavidin Display Assay - Photographing Pellets + TECAN]]<br> | |||
[[Template:SBB- Surface-binding Assay | Surface-binding Assay]]<br> | |||
[[Template:SBB- Growth Rate Assay | Growth Rate Assay]]<br> | |||
[[Template:SBB- Flocculation Assay | Flocculation Assay]]<br> | |||
=== | ===Assembly protocols=== | ||
[[Template:Assemby Protocol | Assembly Protocol]]<br> | |||
===Miniprep Protocols=== | |||
[[Template:SBB-Protocols_Micro4 | Machery-Nagel Miniprep purification of DNA]]<br> | |||
[[Template:SBB-Protocols_Mini1 | Quiagen Miniprep purification of DNA]]<br> | |||
[[Template:SBB-Protocols_Mini2 | Quiagen 8-strip Miniprep purification of DNA]]<br> | |||
Latest revision as of 16:33, 14 August 2009
|
University of California Berkeley iGem 2009 Wetlab Team |
|---|
All these protocols in one big list
Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
PCA gene synthesis
Colony PCR
Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification
Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)
EcoRI/BamHI Digest of vector plasmid
Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Other
LR Gateway Transfers
What do I do with my sequencing data?
In Vivo Gateway
EcoRI/BamHI Transfer
Reaction Guides
SOEing with ProTips
Colony PCR
Binding/Display assay protocols
Streptavidin Display Assay - Microscopy - do longer in development
Streptavidin Display Assay - TECAN
Streptavidin Display Assay - ELESA - no longer in development
Streptavidin Display Assay - Photographing Pellets + TECAN
Surface-binding Assay
Growth Rate Assay
Flocculation Assay
Assembly protocols
Miniprep Protocols
Machery-Nagel Miniprep purification of DNA
Quiagen Miniprep purification of DNA
Quiagen 8-strip Miniprep purification of DNA
