UCBigem09-Protocols: Difference between revisions
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[[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br> | [[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br> | ||
===Full Reaction Cycles=== | ===Full Reaction Cycles with CliffNotes=== | ||
[[Template:SBB-SOEing | SOEing]]<br> | [[Template:SBB-SOEing | SOEing with CliffNotes]]<br> | ||
===Functional assay protocols=== | ===Functional assay protocols=== | ||
Revision as of 16:05, 25 June 2009
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University of California Berkeley iGem 2009 Wetlab Team |
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All these protocols in one big list
Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
Template:SBB-Protocols_PCR4 ! PCA gene synthesis
Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification
Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)
EcoRI/BamHI Digest of vector plasmid
Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Machery-Nagel Miniprep purification of DNA
Other
LR Gateway Transfers
What do I do with my sequencing data?
Full Reaction Cycles with CliffNotes
Functional assay protocols
Streptavidin Display Assay - Microscopy
Streptavidin Display Assay - TECAN
Streptavidin Display Assay - ELESA
