UCBigem09-groupmtg: Difference between revisions
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Topics covered:<br> | Topics covered:<br> | ||
*cellulases - Azo Cm assay data discussed. | *cellulases - Azo Cm assay data (which can be found [http://openwetware.org/wiki/Image:9-3-09_cellulase_assay_2.xls here]) discussed. | ||
-cel5B data looks too | -cel5B data looks too good since most of the constructs had signal<br> | ||
-cel3A had readings close to the negative controls <br> | |||
-cel9A had some signals that are significant, but not high<br> | |||
-some next steps: 1. repeat assay with positive control (which we got from Lane) <br> 2. make constructs without the displayers to test whether the cellulases have function without displayers<br> | |||
*BCA(?) assay on mgfp | |||
-will run the assay again<br> | |||
-if no improvement seen, will go with flocculation | |||
*Needle scFv - will FITC conjugate today and will assay tomorrow | |||
*assembling of parts - mgfp with linkers have been made. Will make cellulases with linkers, needle scFV with linkers, and internal passengers. New stock plates of cellulases are being made. | |||
*Area of focus | |||
-currently - fundamental. highlighting: autotransporters, interesting displayed proteins (scFv, enzyme, structural, peptide zippers), automation, number of parts, characterization/improvement/preliminary rules of cell surface display (linkers and displayers) as a tool for future surface display endeavors. | |||
*Team names, logos, clever quotes, and other sorts of ridiculous nonscience. | |||
Terry: What type of shirts do you guys want for the team? T-shirts, polos... | |||
Jenn: Let's get halters! | |||
"think outside the cell" -Pat | |||
Date: 7/9/09 <br> | Date: 7/9/09 <br> | ||
Presentation: Basic Parts update, Strep Assay, and Functional Assays (Susan, Gaby, Patrick) <br> | Presentation: Basic Parts update, Strep Assay, and Functional Assays (Susan, Gaby, Patrick) <br> | ||
[[Media:7_9_09 presentation slides.ppt]] | [[Media:7_9_09 presentation slides.ppt]] | ||
Revision as of 20:42, 4 September 2009
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University of California Berkeley iGem 2009 Wetlab Team |
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Friday 9-4-09 Meeting Minutes
Topics covered:
- cellulases - Azo Cm assay data (which can be found here) discussed.
-cel5B data looks too good since most of the constructs had signal
-cel3A had readings close to the negative controls
-cel9A had some signals that are significant, but not high
-some next steps: 1. repeat assay with positive control (which we got from Lane)
2. make constructs without the displayers to test whether the cellulases have function without displayers
- BCA(?) assay on mgfp
-will run the assay again
-if no improvement seen, will go with flocculation
- Needle scFv - will FITC conjugate today and will assay tomorrow
- assembling of parts - mgfp with linkers have been made. Will make cellulases with linkers, needle scFV with linkers, and internal passengers. New stock plates of cellulases are being made.
- Area of focus
-currently - fundamental. highlighting: autotransporters, interesting displayed proteins (scFv, enzyme, structural, peptide zippers), automation, number of parts, characterization/improvement/preliminary rules of cell surface display (linkers and displayers) as a tool for future surface display endeavors.
- Team names, logos, clever quotes, and other sorts of ridiculous nonscience.
Terry: What type of shirts do you guys want for the team? T-shirts, polos... Jenn: Let's get halters!
"think outside the cell" -Pat
Date: 7/9/09
Presentation: Basic Parts update, Strep Assay, and Functional Assays (Susan, Gaby, Patrick)
Media:7_9_09 presentation slides.ppt
