User:A. Florian Sessler

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A. Florian Sessler (an artistic interpretation)
A. Florian Sessler (an artistic interpretation)

I am a member of the iGEM 2010 twam of Imperial College London. I learned about OpenWetWare from the project organiser of my team who told us about this site which we will be using during the following months.

Education

Currently doing my bachelor in Biology with Microbiology at Imperial College London.

Cell State Reporters

Monitoring Cell Activity might be very useful for scientists in many situations. We propose the use of Cell State Reporters to quickly give visual information of the activities or phase the cells are undergoing which could be achieved by using elements that control expression of state specific genes. This might help to maintain a healthy population and to determine whether population grows anaerobically or aerobically. Furthermore it could be used as a warning signal when cells undergo undesired/stressful phases and to examine if the cells grow in synchrony. Many different regulon systems could potentially exploited for this purpose including without limitation:

  • Ntr and Nif system that detect ammonia limitation,
  • Arc and Fnr system that detect anaerobic conditions,
  • the SOS and response to UV light,
  • sporulation in response to starvation and the heat shock response.

Use of system specific promoter, enhancer or repressor elements could be used to create a system that gives feed back in form of different fluorescent proteins to the degree to which the system is activated and by extension about the environment the cell is in.

Control of anaerobic respiration

Arc system

References:

  • Malpica et al., 2006. Signaling by the Arc two-component system provides a link between the redox state of the quinone pool and gene expression. Antioxidants and Redox Signalling, 8, 781-795
  • Iuchi S & Lin EC. 1988. arcA (dye), a global regulatory gene in Escherichia coli mediating repression of enzymes in aerobic pathways. Proc Natl Acad Sci USA 85: 1888–1892,

Fnr system

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