User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/08/31: Difference between revisions

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==Bench Work==
==Bench Work==
* weighed out HAuCl4 (MW 339.79 g/mol) to make a stock solution of 0.010 M
* weighed out [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] (MW 339.79 g/mol) to make a stock solution of 0.010 M
--> weighing paper 0.2688 g <br>
--> weighing paper 0.2688 g <br>
-->weighing paper plus HAuCl4 0.3063 g<br>
-->weighing paper plus HAuCl<sub>4</sub> 0.3063 g<br>
-->weighing paper after 0.277 g<br>
-->weighing paper after 0.277 g<br>
total HAuCl4 0.00285 g was added to 5 mL of water to make a stock solution of 0.0168 M HAuCl4 <br>
0.00285 g HAuCl<sub>4</sub>  was added to 5 mL of water to make a stock solution of 0.0168 M HAuCl<sub>4</sub> <br>


* weighed out BSA (MW 66430 g/mol) to make a stock solution of 15 μM
* weighed out [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] (MW 66430 g/mol) to make a stock solution of 15 μM
-->weighing paper 0.2718 g <br>
-->weighing paper 0.2718 g <br>
-->weighing paper plus BSA 0.2938 g <br>
-->weighing paper plus BSA 0.2938 g <br>
-->weighing paper after 0.2720 g <br>
-->weighing paper after 0.2720 g <br>
total BSA 0.0218 g was added to 20 mL of water to make a stock solution of 0.1.64e-5 M BSA <br>
0.0218 g of BSA was added to 20 mL of water to make a stock solution of 0.1.64e-5 M BSA <br>


*made Au/BSA solutions at 14 different ratios as calculated in the following spreadsheet:
*made 10 mL Au/BSA solutions at 14 different ratios as calculated in the following spreadsheet:


[[Image:20120831 AuNPsynthesisvolumes.png]]
[[Image:20120831 AuNPsynthesisvolumes.png]]
*The solutions were placed at 85 °C at 4:20 pm and heated for 4 hours then cooled to room temperature over 30 minutes
==Data/Spectra==
==Data/Spectra==
* N/A
* N/A
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==Notes==
==Notes==
* assisted by Eleni
* assisted by Eleni
*The HAuCl<sub>4</sub> had to be weighed out using a metal spatula wrapped in parafilm to prevent contact between the choloric acid and the metal.


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Revision as of 14:03, 31 August 2012

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Objective

  • synthesize AuNPs at various ratios to confirm ratio point of aggregation

Bench Work

  • weighed out HAuCl4 (MW 339.79 g/mol) to make a stock solution of 0.010 M

--> weighing paper 0.2688 g
-->weighing paper plus HAuCl4 0.3063 g
-->weighing paper after 0.277 g
0.00285 g HAuCl4 was added to 5 mL of water to make a stock solution of 0.0168 M HAuCl4

  • weighed out BSA (MW 66430 g/mol) to make a stock solution of 15 μM

-->weighing paper 0.2718 g
-->weighing paper plus BSA 0.2938 g
-->weighing paper after 0.2720 g
0.0218 g of BSA was added to 20 mL of water to make a stock solution of 0.1.64e-5 M BSA

  • made 10 mL Au/BSA solutions at 14 different ratios as calculated in the following spreadsheet:

  • The solutions were placed at 85 °C at 4:20 pm and heated for 4 hours then cooled to room temperature over 30 minutes

Data/Spectra

  • N/A

Notes

  • assisted by Eleni
  • The HAuCl4 had to be weighed out using a metal spatula wrapped in parafilm to prevent contact between the choloric acid and the metal.



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