User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/08/31: Difference between revisions

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total BSA 0.0218 g was added to 20 mL of water to make a stock solution of 0.1.64e-5 M BSA <br>
total BSA 0.0218 g was added to 20 mL of water to make a stock solution of 0.1.64e-5 M BSA <br>


*
*made Au/BSA solutions at 14 different ratios as calculated in the following spreadsheet:
 
[[Image:20120831 AuNPsynthesisvolumes.png]]
==Data/Spectra==
==Data/Spectra==
* N/A
* N/A

Revision as of 13:55, 31 August 2012

CHEM571 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  • synthesize AuNPs at various ratios to confirm ratio point of aggregation

Bench Work

  • weighed out HAuCl4 (MW 339.79 g/mol) to make a stock solution of 0.010 M

--> weighing paper 0.2688 g
-->weighing paper plus HAuCl4 0.3063 g
-->weighing paper after 0.277 g
total HAuCl4 0.00285 g was added to 5 mL of water to make a stock solution of 0.0168 M HAuCl4

  • weighed out BSA (MW 66430 g/mol) to make a stock solution of 15 μM

-->weighing paper 0.2718 g
-->weighing paper plus BSA 0.2938 g
-->weighing paper after 0.2720 g
total BSA 0.0218 g was added to 20 mL of water to make a stock solution of 0.1.64e-5 M BSA

  • made Au/BSA solutions at 14 different ratios as calculated in the following spreadsheet:

Data/Spectra

  • N/A

Notes

  • assisted by Eleni


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