User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/09/10: Difference between revisions
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==Objective== | ==Objective== | ||
* | * make stock solutions of HAuCl4 and BSA. | ||
==Bench Work== | ==Bench Work== | ||
* | * HAuCl4, using a parafilm wrapped spatula | ||
--> 0.2680 m paper<br> | |||
--> 0.2753 g HAuCl4 and paper<br> | |||
rinsed into 15 mL opaque falcontube with 10 mL of water (by micropipet) makes 2.15e-3M HAuCl4<br> | |||
*BSA, pour onto paper | |||
--> 0.2778 g paper <br> | |||
--> 0.3177 g paper and BSA<br> | |||
--> 0.2774 paper after<br> | |||
dissolved in 25 mL of water in a 50mL falcon tube makes 3.03e-5 M BSA<br> | |||
*made two ratios of gold to BSA, 80 and 160 | |||
** 80 HAuCl4/BSA: 0.2234 mL of 2.15e-3M HAuCl4, 0.198 mL of 3.03e-5 M BSA, plus 5.579 mL water | |||
** 160 HAuCl4/BSA: 0.4468 mL of 2.15e-3M HAuCl4, 0.198 mL of 3.03e-5 M BSA, plus 5.355 mL water | |||
*heated in oven at 85°C for four hours | |||
==Data/Spectra== | ==Data/Spectra== | ||
* | * N/A | ||
==Notes== | ==Notes== | ||
* | * showed color change after 1 hour | ||
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Revision as of 14:11, 10 September 2012
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Objective
Bench Work
--> 0.2680 m paper
--> 0.2778 g paper
Data/Spectra
Notes
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