User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08

From OpenWetWare

< User:Abigail E. Miller | Notebook | CHEM571 Lab Project | 2012 | 10(Difference between revisions)
Jump to: navigation, search
(Autocreate 2012/10/08 Entry for User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project)
Current revision (13:57, 8 October 2012) (view source)
(Bench Work)
 
(6 intermediate revisions not shown.)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Objective==
==Objective==
-
* Insert content here...
+
* To create standard solution of gold for calibration of the atomic absorption spectrometer. The students will measure the gold content of their BSA nucleated AuNPs against these standards.
==Bench Work==
==Bench Work==
-
* Insert content here...
+
* 1000 ± 10μg of Au/mL of solution ( 10 % v/v HCl) for the stock solution
 +
** from Inorganic Ventures cat# AAAU1-1, lot # F2-AU04012
 +
** the solution is certified by ICP (ion couple plasma spectroscopy) to NIST  SRM 3121
 +
 
 +
For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance) for this instrument. recent experiments put the detection limit closer to 2 ppm for gold, and since 20 ppm is roughly 0.1 mM Au, therefore I created standards ranging from 5 to 40 ppm.
 +
 
 +
For reference the molecular weight of gold is 197 g/mol.
 +
 
 +
* created 50 mL of each standard: 5, 8, 10, 15, 20, 25, 30, 40 ppm Au
 +
**from the 1000 ppm Au stock standard
 +
** using 50 mL volumetric flasks
 +
** all diluted in 1 M HCl
 +
{| {{table}}
 +
| align="center" |'''ppm'''
 +
| align="center" |'''mL of 1000 ppm stock added'''
 +
|-
 +
| 5||0.25
 +
|-
 +
| 8||0.40
 +
|-
 +
| 10||0.50
 +
|-
 +
| 15||0.75
 +
|-
 +
| 20||1.00
 +
|-
 +
| 25||1.25
 +
|-
 +
| 30||1.50
 +
|-
 +
| 40||2.00
 +
|-
 +
|
 +
|}
==Data/Spectra==
==Data/Spectra==
-
* Insert content here...
+
* N/A - solutions will be used for AAS on 10/9/2012
==Notes==
==Notes==
-
* Insert content here...
+
* N/A
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

CHEM571 Main project page
Previous entry      Next entry

Objective

  • To create standard solution of gold for calibration of the atomic absorption spectrometer. The students will measure the gold content of their BSA nucleated AuNPs against these standards.

Bench Work

  • 1000 ± 10μg of Au/mL of solution ( 10 % v/v HCl) for the stock solution
    • from Inorganic Ventures cat# AAAU1-1, lot # F2-AU04012
    • the solution is certified by ICP (ion couple plasma spectroscopy) to NIST SRM 3121

For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance) for this instrument. recent experiments put the detection limit closer to 2 ppm for gold, and since 20 ppm is roughly 0.1 mM Au, therefore I created standards ranging from 5 to 40 ppm.

For reference the molecular weight of gold is 197 g/mol.

  • created 50 mL of each standard: 5, 8, 10, 15, 20, 25, 30, 40 ppm Au
    • from the 1000 ppm Au stock standard
    • using 50 mL volumetric flasks
    • all diluted in 1 M HCl
ppm mL of 1000 ppm stock added
50.25
80.40
100.50
150.75
201.00
251.25
301.50
402.00

Data/Spectra

  • N/A - solutions will be used for AAS on 10/9/2012

Notes

  • N/A


Personal tools