User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08

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(Bench Work)
(Bench Work)
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==Bench Work==
==Bench Work==
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* 1000 ± 10μg of Au/mL of H<sub>2</sub>O stock solution
+
* 1000 ± 10μg of Au/mL of solution ( 10 % v/v HCl) for the stock solution
** from Inorganic Ventures cat# AAAU1-1, lot # F2-AU04012
** from Inorganic Ventures cat# AAAU1-1, lot # F2-AU04012
** the solution is certified by ICP (ion couple plasma spectroscopy) to NIST  SRM 3121
** the solution is certified by ICP (ion couple plasma spectroscopy) to NIST  SRM 3121
Line 16: Line 16:
For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance)for this instrument. recent experiment is closer to 2 ppm and since 20 ppm is roughly 0.1 mM Au, Therefore I created standards ranging from 5 to 40 ppm.
For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance)for this instrument. recent experiment is closer to 2 ppm and since 20 ppm is roughly 0.1 mM Au, Therefore I created standards ranging from 5 to 40 ppm.
-
* created 50 mL of each standard: 5, 8, 10, 15, 20, 25, 30, 40 ppm Au from the 1000 ppm Au stock standard.
+
* created 50 mL of each standard: 5, 8, 10, 15, 20, 25, 30, 40 ppm Au  
-
**
+
**from the 1000 ppm Au stock standard
 +
** using 50 mL volumetric flasks
 +
** all diluted in 1 M HCl
{| {{table}}
{| {{table}}
| align="center" |'''ppm'''
| align="center" |'''ppm'''

Revision as of 13:51, 8 October 2012

CHEM571 Main project page
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Objective

  • To create standard solution of gold for calibration of the atomic absorption spectrometer. The students will measure the gold content of their BSA nucleated AuNPs against these standards.

Bench Work

  • 1000 ± 10μg of Au/mL of solution ( 10 % v/v HCl) for the stock solution
    • from Inorganic Ventures cat# AAAU1-1, lot # F2-AU04012
    • the solution is certified by ICP (ion couple plasma spectroscopy) to NIST SRM 3121

For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance)for this instrument. recent experiment is closer to 2 ppm and since 20 ppm is roughly 0.1 mM Au, Therefore I created standards ranging from 5 to 40 ppm.

  • created 50 mL of each standard: 5, 8, 10, 15, 20, 25, 30, 40 ppm Au
    • from the 1000 ppm Au stock standard
    • using 50 mL volumetric flasks
    • all diluted in 1 M HCl
ppm mL of 1000 ppm stock added
50.25
80.40
100.50
150.75
201.00
251.25
301.50
402.00

Data/Spectra

  • Insert content here...

Notes

  • Insert content here...


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