User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08: Difference between revisions
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==Objective== | ==Objective== | ||
* | * To create standard solution of gold for calibration of the atomic absorption spectrometer. The students will measure the gold content of their BSA nucleated AuNPs against these standards. | ||
==Bench Work== | ==Bench Work== | ||
* | * 1000 ± 10μg of Au/mL of solution ( 10 % v/v HCl) for the stock solution | ||
** from Inorganic Ventures cat# AAAU1-1, lot # F2-AU04012 | |||
** the solution is certified by ICP (ion couple plasma spectroscopy) to NIST SRM 3121 | |||
For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance) for this instrument. recent experiments put the detection limit closer to 2 ppm for gold, and since 20 ppm is roughly 0.1 mM Au, therefore I created standards ranging from 5 to 40 ppm. | |||
For reference the molecular weight of gold is 197 g/mol. | |||
* created 50 mL of each standard: 5, 8, 10, 15, 20, 25, 30, 40 ppm Au | |||
**from the 1000 ppm Au stock standard | |||
** using 50 mL volumetric flasks | |||
** all diluted in 1 M HCl | |||
{| {{table}} | |||
| align="center" |'''ppm''' | |||
| align="center" |'''mL of 1000 ppm stock added''' | |||
|- | |||
| 5||0.25 | |||
|- | |||
| 8||0.40 | |||
|- | |||
| 10||0.50 | |||
|- | |||
| 15||0.75 | |||
|- | |||
| 20||1.00 | |||
|- | |||
| 25||1.25 | |||
|- | |||
| 30||1.50 | |||
|- | |||
| 40||2.00 | |||
|- | |||
| | |||
|} | |||
==Data/Spectra== | ==Data/Spectra== | ||
* | * N/A - solutions will be used for AAS on 10/9/2012 | ||
==Notes== | ==Notes== | ||
* | * N/A | ||
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Revision as of 11:57, 8 October 2012
 CHEM571
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Objective
Bench Work
For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance) for this instrument. recent experiments put the detection limit closer to 2 ppm for gold, and since 20 ppm is roughly 0.1 mM Au, therefore I created standards ranging from 5 to 40 ppm. For reference the molecular weight of gold is 197 g/mol.
Data/Spectra
Notes
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