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 CHEM571
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Objective
- To create standard solution of gold for calibration of the atomic absorption spectrometer. The students will measure the gold content of their BSA nucleated AuNPs against these standards.
Bench Work
- 1000 ± 10μg of Au/mL of solution ( 10 % v/v HCl) for the stock solution
- from Inorganic Ventures cat# AAAU1-1, lot # F2-AU04012
- the solution is certified by ICP (ion couple plasma spectroscopy) to NIST SRM 3121
For the AAS (atomic absorption Spectrometer), the published detection limit for gold is 0.2 ppm ( a 1% absorbance)for this instrument. recent experiment is closer to 2 ppm and since 20 ppm is roughly 0.1 mM Au, Therefore I created standards ranging from 5 to 40 ppm.
- created 50 mL of each standard: 5, 8, 10, 15, 20, 25, 30, 40 ppm Au
- from the 1000 ppm Au stock standard
- using 50 mL volumetric flasks
- all diluted in 1 M HCl
| ppm
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mL of 1000 ppm stock added
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| 5 |
0.25
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| 8 |
0.40
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| 10 |
0.50
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| 15 |
0.75
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| 20 |
1.00
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| 25 |
1.25
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| 30 |
1.50
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| 40 |
2.00
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Data/Spectra
- N/A - solutions will be used for AAS on 10/9/2012
Notes
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CHEM571
