User:Adriana De Castro/Notebook/Biology 210 at AU

Adriana De Castro Prof. Moriarty Bio 210-009 28 January 2015 Microorganisms in my Hay Infusion I. Introduction: In this experiment we are going to observe samples of our hay infusion to identify protist found in that ecosystem. We want to observe their characteristics and try to differentiate algae and protist by using a Dichotomous Key to identify the exact organism. II. Methods and Materials: In this experiment we made a wet mount of organisms we already knew about and then used the Dichotomous Key to determine that organisms identity that was know to practice and learn how to use the Dichotomous Key. As a group we then took our Hay Infusion and made three wet mounts one from the top niche, one from the middle niche, and one from the bottom niche. After we observed them on the microscope and identified one organism from each niche in the hay infusion. Later we prepared the serial dilutions that are going to be observed in the next lab. We labeled four tubes of 10mLs sterile broth 10-2, 10-4, 10-6, 10-8. We took eight plates four with nutrient agar and four nutrient agar plus tetracycline and we labeled one plate from each group with 10-3, 10-5, 10-7, 10-9. We pipetted 100micro/liters from the Hay Infusion to the 10mLs of sterile broth into the tube labeled10-2. Another 100micro/liters of broth were taken from tube 10-2 and were passed to the tube labeled 10-4 and the same procedure was done to make the 10-6 and 10-8 serial dilutions. To create the serial dilutions on the plate we pipetted 100micro/liters from the 10-2 tube and placed them in the agora plate labeled 10-3 and was spread. The exact procedure was made for the three remaining plates. The 100micro/liters from the 10-4 tube were placed on the plate labeled 10-5, the 10-6 100micro/liters were placed in the plate labeled 10-7 and the same amount also was taken from 10-8 and placed on the 10-9 plates. These serial dilutions were left in the rack for one week to develop. III. Results: The Hay Infusion culture smelled like pool water however it had like mold in the surface. From the three different niches we took a sample from we found different organism. According to the Dichotomous Key we found in the top niche we found a Blepharisma . This paramecium had a light pink coloring inside and measured about 206micro/liters. In the middle niche we found an Euglena that had a long shape with green coloring and measured about 37micro/liters. In the bottom niche we believed we found a Paramecium bursaria. This paramecium had a rounded shape and was very motile, crawling under the dirt seen in the slide. The serial dilution made for the agora plates was left incubating and the results if any will be seen next week. IV. Data and Observations: Serial Dilution

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Hay Infusion

Hay Infusion

Euglena

Lab notes drawings about organisms we saw in the top and last level and we couldn’t take pictures off.