User:Ai Oikawa

Ai Oikawa

Lawrence Berkeley National Laboratory
DOE Joint BioEnergy Institute
Buiding 978 Mailstop 4121, CA 94720
Room: 4450
http://jbei.org/
Postdoctoral Fellow
Feedstocks Division
Office: 510 495 2462
AOikawa@lbl.gov

Education and experience

• 2008-Current Postdoctoral Fellow: Joint BioEnergy Institute, California, USA
• 2005-2008 Ph.D: Iwate University, JAPAN
• 2007 Aug-Sep Visiting student: Yale University, Connecticut, USA
• 2003-2005 M.S: Iwate University, JAPAN
• 1998-2003 B.S: Iwate University, JAPAN

Awards and Grants received

2008

• The Yamada Science Foundation Japan (March 2008): I was awarded the Yamada science abroad research grant to study on “Unraveling the gene network involved in the construction of plant cell wall” (CA, USA).

2007

• The Japanese Society of Plant Physiologists Fellow (August-September 2007): I was selected for the JSPP Fellowship as a JSPP Fellow for the year 2007 to conduct collaboration research with Dr. Timothy Nelson at Yale University (CT, USA) on “Unraveling the gene network involved in the barley seed development by LCM (Laser-captured microdissection)“.
• The Life Science Foundation Japan (only japanese) (July 2007): I was awarded the life science travel grant to attend the Annual meeting of American Society of Plant Biologist (ASPB) held in Chicago, USA.

Publications

2007

• Ai Oikawa, Abidur Rahman, Tetsuro Yamashita, Hideharu Taira and Shin-ichiro　Kidou. (2007) Virus-induced gene silencing of P23k in barley leaf reveals morphological changes involved in secondary wall formation. Journal of Experimental Botany 58:2617-2625 Paper
• Ai Oikawa, Tetsuro Yamashita, Hideharu Taira, Shin-ichiro Ejiri and Shin-ichiro Kidou. (2007) Jasmonate-induced 23kD protein (JIP-23) is involved in seed development of barley. Plant Biotechnology 24:217-220 Paper

2006

• Shin-ichiro Kidou, Ai Oikawa, Naoko Sasaki, Hiroshi Yasuda, Tetsuro Yamashita, Hiroyuki Koiwa, Kiyoaki Kato, Shin-ichiro Ejiri. (2006) Identification of a 23 KD protein (P23k) related to the sugar supply in germinating seed. Plant Biotechnology 23:357-364 Paper

Presentations at National/International meeting (2005-)

2009

• Ai Oikawa, Anongpat Suttangkakul, Naomi Geshi, Henrik Vibe Scheller. Development of high throughput screening of glycosyltransferases.(2009) The Gordon Research Conference (GRC) on Plant Cell Wall (Smithfield, Rich island)
• Ai Oikawa, Naomi Gesh, Henrik Vibe Scheller.A comprehensive bioinformatics/biochemical approach to identify key cell wall glycosyltransferase for biofuel.(2009) Synthetic biology workshop by the Danish Ministry of Science (Berkeley, California)
• Anongpat Suttangkakul, Ai Oikawa, Michelle Truong, Yuzuki Manabe,Henrik Vibe Scheller. Functional Characterization of Arabidopsis Glycosyltransferases.(2009) Annual Meeting of the American Society for Plant Biology (Oahu, Hawaii)
• Ai Oikawa, Naomi Geshi, Henrik Vibe Scheller. TRBO-GT screen: Tobacco mosaic virus RNA Based Overexpressed-GlycosylTransferase assay and its application into 96 well format.(2009) Annual Meeting of the American Society for Plant Biology (Oahu, Hawaii)
• Henrik Vibe Scheller, Ai Oikawa, Lan Yin, Eva Knoch, Naomi Geshi, Carsten Rautengarten, Yuzuki Manabe, and Chithra Manisseri.Systematic Characterization of Glycosyltransferases Involved in Plant Cell Wall Biosynthesis.(2009) Genomics:GTL Awardee Workshop VII and USDA-DOE Plant Feedstock Genomics for Bioenergy Awardee Workshop (Bethesda, Maryland)

2008

• Naomi Geshi, Ai Oikawa, Lan Yin, Mohammed Saddik Motawia, Henrik Vibe Scheller. Glycosyltranfserases involved in the plant cell wall polysaccharides biosynthesis.(2008) Annual Meeting of the Society for Glycobiology (Fort Worth, Texas)
• Henrik Vibe Scheller, Ai Oikawa, Yuzuki Manabe, Jorunn Johansen, Herman Höfte, Mohammed Saddik Motawia, Naomi Geshi. (2008) Cell Wall Biosynthesis: Heterologous expression of Arabidopsis glycosyltransferases in Nicotiana benthamiana. XVI Congress of the Federation of European Societies of Plant Biology (Tampere, Finland)

2007

• Ai Oikawa, and Shin-ichiro Kidou (2007). Functional analysis of the barley P23k protein by virus-induced gene silencing. The 5th international symposium of rice functional genomics (Tsukuba, Japan)
• Ai Oikawa., Abidur Rahman and Shin-ichiro Kidou(2007). Virus-induced gene silencing of P23k in barley leaf reveals morphological changes　involved in secondary wall formation. Plant Biology & Botany 2007 joint congress (Chicago, illinois, USA)

2006

• Ai Oikawa, Aiko Uemura and Shin-ichiro Kidou (2006). Jasmonate-induced 23kD protein (JIP-23) is involved in seed development of barley. Iwate international Plant Science Symposium 2006 (Iwate, Japan)　
• Ai Oikawa, and Shin-ichiro Kidou(2006). P23k relates to the sugar supply at various developmental stages in barley. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress (Kyoto, Japan)
• Kyouhei Shibasaki, Ai Oikawa and Shin-ichiro Kidou (2006). Identification of a novel barley 23kDa protein (P23k) related to the sugar supply in germinating seeds. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress (Kyoto, Japan)

2005

• Ai Oikawa, and Shin-ichiro Kidou (2005). A barley 23kDa protein which is up-regulated by sugars at various developmental stages. 10th International Congress of SABRAO (Tsukuba, Japan)
• Ai Oikawa, and Shin-ichiro Kidou (2005). Expression of a 23kDa protein is up-regulated by sugars at various developmental stages in barley.　The Gordon Research Conference (GRC) on Temperature Stress in Plants　(Ventura, California, USA)

Professional Affiliations

• American Society of Plant Biologists
• Japanese Society of Plant Physiologist
• Scandinavian Plant Physiology Society

Expertise and Skills

• DNA, RNA, and Protein analysis: DNA cloning and sequencing, RNA isolation and mRNA purification, RT-PCR, Northern blot, Protein extraction, Native-PAGE, SDS-PAGE, Western blotting, Protein and antibody purification using affinity chromatography, Enzyme assay measurement
• Histochemistry analysis: In situ hybridization, Serial sectioning immunohistochemistry, Serial sectioning saccharide stains techniques
• Intracellular localization: Immunofluorescence cytochemistry, Immnoelectoro-cytochemistory, Cell fractionation
• Microscopy:Light microscopy, Fluorescent microscopy, Electron microscopy
• Protein-protein interaction: Affinity of protein expression in E.coli, GST-pull downs, BIA-CORE, Yeast two hybrid system
• Gain or loss of function: Plant cell cultures, Agrobacterium transfection, Over-expression and RNAi, Floral dip methods in Arabidopsis, Virus-induced gene silencing (by BSMV in monocots)
• Plant model systems: Arabidopsis, Tobacco, Rice, Barley