User:Ai Oikawa

Ai Oikawa

Lawrence Berkeley National Laboratory
DOE Joint BioEnergy Institute
Buiding 978 Mailstop 4121, CA 94720
Room: 4450
http://jbei.org/
Postdoctoral Fellow
Feedstocks Division
Office: 510 495 2462
AOikawa@lbl.gov

Expertise and Skills

• DNA, RNA, and Protein analysis -- DNA cloning and sequencing, RNA isolation and mRNA purification, RT-PCR, Northern blot, Protein extraction, Native-PAGE, SDS-PAGE, Western blotting, Protein and antibody purification using affinity chromatography
• Lipid composition analysis -- Lipid extraction, TLC analysis, Gas Chromatography
• Histochemistry analysis -- Histochemistry analysis In situ hybridization, Serial sectioning immunohistochemistry, Serial sectioning saccaride stains techniques
• Intracellular localization -- Immunofluorescence cytochemistry, Immnoelectoro-cytochemistory, Cell fractionation, Enzyme assay measurement
• Microscopy -- Light microscopy, Fluorescent microscopy, Electron microscopy
• Protein-protein interaction -- Affinity of protein expression in E.coli, GST-pull downs, BIA-CORE, Yeast two hybrid system
• Gain or loss of function -- Plant cell cultures, Agrobacterium transfection, Over-expression and RNAi, Floral dip methods in Arabidopsis, barley Virus-Induced Gene Silencing (by barley stripe mosaic virus)
• Plant model systems -- Arabidpsis, Rice, Barley, Monocot callus

Awards

2008

• The Yamada Science Foundation Japan (March 2008) I was awarded the Yamada science abroad research grant to study on “Unraveling the gene network involved in the construction of cell wall” in CA, USA.

2007

• The Japanese Society of Plant Physiologists Fellow (July- Aug 2007) I was awarded the JSPP Fellow for the year 2007 to conduct a collaboration research with Dr. Timothy Nelson of Yale University (CT, USA) on "Unraveling the gene network involved in the barley seed development by LCM（Laser-captured microdissection)".
• The Life Science Foundation Japan (only japanese) (July 2007) I was awarded the life science travel grant to attend the Annual meeting of American Society of Plant Biologist (ASPB) -2007 held in Chicago, USA.

Publications

2007

• Ai Oikawa, Abidur Rahman, Tetsuro Yamashita, Hideharu Taira and Shin-ichiro　Kidou. (2007) Virus-induced gene silencing of P23k in barley leaf reveals morphological changes involved in secondary wall formation. Journal of Experimental Botany 58:2617-2625 Paper (web)
• Ai Oikawa, Tetsuro Yamashita, Hideharu Taira, Shin-ichiro Ejiri and Shin-ichiro Kidou. (2007) Jasmonate-induced 23kD protein (JIP-23) is involved in seed development of barley. Plant Biotechnology 24:217-220 Paper (pdf)

2006

• Shin-ichiro Kidou, Ai Oikawa, Naoko Sasaki, Hiroshi Yasuda, Tetsuro Yamashita, Hiroyuki Koiwa, Kiyoaki Kato, Shin-ichiro Ejiri. (2006) Identification of a 23 KD protein (P23k) related to the sugar supply in germinating seed. Plant Biotechnology 23:357-364 Paper (pdf)

Posters presented in International meetings

2007

• Oikawa, A., and Kidou, S. (2007) Functional analysis of the barley P23k protein by virus-induced gene silencing. The 5th international symposium of rice functional genomics (Tsukuba, Japan)
• Oikawa, A., Rahman A and Kidou, S. (2007) Virus-induced gene silencing of P23k in barley leaf reveals morphological changes　involved in secondary wall formation. Plant Biology & Botany 2007 joint congress (Chicago, illinois, USA)

2006

• Oikawa, A., Uemura A and Kidou, S. (2006). Jasmonate-induced 23kD protein (JIP-23) is involved in seed development of barley. Iwate international Plant Science Symposium 2006 (Iwate, Japan)　
• Oikawa, A., and Kidou, S. (2006). P23k relates to the sugar supply at various developmental stages in barley. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress (Kyoto, Japan)
• Shibasaki, K., Oikawa, A. and Kidou, S. (2006). Identification of a novel barley 23kDa protein (P23k) related to the sugar supply in germinating seeds. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress (Kyoto, Japan)

2005

• Oikawa, A., and Kidou, S. (2005) A barley 23kDa protein which is up-regulated by sugars at various developmental stages. 10th International Congress of SABRAO (Tsukuba, Japan)
• Oikawa, A., and Kidou, S. (2005) Expression of a 23kDa protein is up-regulated by sugars at various developmental stages in barley.　The Gordon Research Conference (GRC) on Temperature Stress in Plants　(Ventura, California, USA)

Professional Affiliations

• American Society of Plant Biologists
• Japanese Society of Plant Physiologist
• Scandinavian Plant Physiology Society

Education

• Ph.D. (2008) Department of Biological Resource Science -- The United Graduate School of Agricultural Sciences, Iwate University, JAPAN.
• M.S. (2005) Department of Biological Resource Science -- Graduate School of Agriculture, Master's Program, Iwate University, JAPAN.
• B.S. (2003) Department of Bioscience and Technology -- Faculty of Agriculture, Iwate University, JAPAN

Personal information

• Nationality -- Japanese
• Birth year -- 1980
• Place of Birth -- Iwate, Japan
  • E-MI-SHI - The original peoples in Iwate.
  • Kenji MIYAZAWA (1896-1933) - He is a scientist, writer, painter, and religionist in Iwate.
  • Inazo NITOBE (1862-1933) - He is a Christian, agricultural economist, author, educator, diplomat, and politician from Iwate. He is also the author of ｢Bushido: The Soul of Japan｣.

Useful links

• Introductory tutorial
• OpenWetWare help pages