User:Alesha B. Castillo

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Contact Information

Alesha B. Castillo, PhD

Postdoctoral Scholar

Biomechanical Engineering

Stanford University

Stanford, CA 94305

Email: alesha.castillo@stanford.edu

Education

• 2004, PhD, Biomedical Engineering, University of California, Davis
• 2000, MS, Biomedical Engineering, University of California, Davis
• 1997, BS, Materials Engineering, California Polytechnic State University, San Luis Obispo

Research Interests

• Bone mechanotransduction, adaptation, aging and disease
• Regenerative medicine and tissue engineering
• Stem cell biology

Research Links

• Projects‎ (under construction)
• Laboratory protocols
• Standard Operating Procedure (SOPs)

Current Funding Sources

• 2007-2010, Stanford Center on Longevity (project information).
• 2007-present, National Institutes of Health (PI: Christopher R. Jacobs, Columbia University, Jacobs Lab Link)

Online Research Tools

• UCSC Genome Browser
• Addgene
• Primer3Plus
• Cre-X-Mice, A Database of Cre Transgenic Lines
• Mouse Genome Informatics (MGI)
• Database of Databases (DBoDB), an easy to use database for searching and retrieving mouse resource information
• Protocols Online Search Engine

Bioresources

• Pubmed
• NCBI Bookshelf
• American Type Culture Collection (ATCC), The Global Bioresource Center
• The Jackson Laboratory
• Mutant Mouse Regional Resources Center (MMRRC)
• Molecular Biology of the Cell by Alberts, et al., New York and London: Garland Science ; c2002

Scientific Journals and Conferences

• Cell Stem Cell, official journal of the International Society for Stem Cell Research (ISSCR)
• Journal of Bone and Mineral Research, official journal of the American Society for Bone and Mineral Research (ASBMR)
• Stem Cells
• Bone, official journal of the International Bone and Mineral Society (IBMS)
• Advances in Mineral Metabolism (AIMM)
• International Bone Fluid Flow (IBFF) Workshop
• Society for Physical Regulation in Biology and Medicine (SPRBM)
• Sun Valley Workshop on Skeletal Tissue Biology

Other Links

• Department of Biomechanical Engineering, Stanford University
• Department of Bioengineering, Stanford University
• Stanford Center on Longevity
• Stanford University Institute for Stem Cell Biology and Regenerative Medicine
• California Institute for Regenerative Medicine (CIRM)
• Howard Hughes Medical Institute (HHMI)
• International Stem Cell Forum (ISCF)
• Nature Reports Stem Cells
• National Institutes of Health (NIH)
• NIH Stem Cell Portal
• The Scientist

FAK Laboratory Protocols (Temporary Placeholder)

• General FAK Project Outline
• Analyzing FAK and Pyk2 in Early Integrin Signaling Events, Current Protocols in Cell Biology
• Thawing Cells
• Subculturing
• Subculturing 3T3 Fibroblasts
• Actin Staining
• Subcloning Outline
• Subcloning Cells
• Hygromycin B Selection
• Transfection of 3T3s
• Read Me: Literature Summary
• Boutahar et al., 2004
• Kim et al., 2007
• Parsons et al., 2007
• Schaller et al., 1996
• Schlaepfer et al., 1999