User:Alexander.wong/sandbox: Difference between revisions

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(New page: frame|Picture This is a picture desc | file 15:00, 12 July 2007 ICGEMS_board1.jpg Dirk Van Swaay 63,548 (testing file) desc | file 14:32, 12 July 2007 Lux_o...)
 
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[[ IMAGE: ICGEMS_board1.jpg|frame|Picture]] This is a picture
==Questions to ask the fluorometer Engineer on Friday, Aug 3==


desc | file 15:00, 12 July 2007 ICGEMS_board1.jpg Dirk Van Swaay 63,548 (testing file)
See if our plate reader has the ability to measure what you want to measure.  
desc | file 14:32, 12 July 2007 Lux_overnight_fluorescence_7_11_07.xls Guido 78,848 
*What are the classes of measurement? Can it do time-resolved fluorometry + Absorbance?
desc | file 13:24, 12 July 2007 PKU_Switch_07-7-12_SDD_EGFP1-12_RD1_13-18_CD1_19-23.jpg Chen Daizhuo 6,338 (l to r: SDD_EGFP_pCC002(1-12), RD1(13-18), CD1(19-23), pMDB, ladder)
*Delay time-resolve - 24 hour experiment; evaporation of solution over long-time courses?!
desc | file 13:19, 12 July 2007 PKU_Switch_07-7-12_SDD'_EGFP-pCC002_digestion_again.jpg Chen Daizhuo 9,992 (l to r: Marker, SDD'_EGFP_pCC002(1-8))
*Temperature-regulation?
desc | file 06:33, 12 July 2007 07.07.11_-colony.JPG Nishant M. Bhat 51,714 
* How do we subtract background values from data and convert from absorbance to OD600?
desc | file 06:28, 12 July 2007 7-7-11-e0240bb.JPG Nishant M. Bhat 61,979 
desc | file 05:40, 12 July 2007 7-7-10.jpg Nishant M. Bhat 57,748 
desc | file 00:49, 12 July 2007 Example_of_an_empty_file.txt Ben Yi Tew 63 
desc | file 00:37, 12 July 2007 My_adorable_sheepy.gif Ben Yi Tew 3,418 
desc | file 23:37, 11 July 2007 My_adorable_sheepy.png Ben Yi Tew 830 (Just a very adorable sheep...)
desc | file 23:07, 11 July 2007 Header1.png Kun Zhang 40,682 
desc | file 20:31, 11 July 2007 07.07.10.jpg Jessica Karen Wong 31,601 
desc | file 20:30, 11 July 2007 2007-07-11_perryColonyPCRgx.jpg GeorgeXu 121,839 
desc | file 19:37, 11 July 2007 I_feed_large.gif Abhishektiwari 1,737 
desc | file 19:03, 11 July 2007 Tripwire.gif Ilya 4,679 
desc | file 18:16, 11 July 2007 Tab_icon.png Ben Yi Tew 1,135 
desc | file 15:39, 11 July 2007 PKU_bear_logo.gif Chen Daizhuo 49,078 (by CXY~)
desc | file 15:30, 11 July 2007 PKU_Switch_07-7-11_SD1-3_ECFP_pCC002.gif Chen Daizhuo 4,937 
desc | file 15:05, 11 July 2007 PKU_Switch_07-7-11_SDD'-EGFP-pCC002_SDD'-EGFP-BamHI-F&pCCtestR.gif Chen Daizhuo 9,981 (SDD'-EGFP-pCC002(13-35), Negative Control, Marker)
desc | file 15:03, 11 July 2007 SS3_SS2_SDD'-EGFP-pCC002_pcr_test.gif Chen Daizhuo 10,793 (l to r: SS3(31-32), SS2(37-44), SDD'-EGFP-pCC002(1-12), none, Negative control for SS2, Marker)
desc | file 15:00, 11 July 2007 SS1_SD3_pcr_test_larger.gif Chen Daizhuo 14,000 (l to r: Negative control, SS1(1-10), SD3(13-18), SS3(25-30), RD1, Marker)
desc | file 14:14, 11 July 2007 Macintosh_HD-Users-nkuldell-Desktop-Enzymes_in_20109lab.doc Nkuldell 19,968 
desc | file 05:51, 11 July 2007 HBNG.pdf Abhishektiwari 183,129 
desc | file 02:38, 11 July 2007 11-07-07_1733.jpg Lucas A. Hadjilucas 42,439 (Test image)
desc | file 20:55, 10 July 2007 Colonypcrpt071007.jpg Shlo 153,008 
desc | file 20:30, 10 July 2007 SB_Primer_100707.pdf Scmohr 10,278,992 (First posted draft (7/10/07))
desc | file 20:26, 10 July 2007 Colonypcrgx071007.jpg Shlo 171,241 
desc | file 20:22, 10 July 2007 Andrew_hessel.JPG Ahessel 41,270 
desc | file 20:14, 10 July 2007 Colonypcrsl0710073min.jpg Shlo 156,834 
desc | file 18:46, 10 July 2007 ThumbWiki.png KMagic246 39,027 
desc | file 18:39, 10 July 2007 Webpage.png KMagic246 106,781 


Talk about using the plate reader and creating protocols.
*Excitation filters and Emission filters for GFP, acGFP and DsRed-Express?
*Any manufacturer protocols for plate reader usage?
*Well plate rows and columns? How is data arranged sequentially with the wells?
*What kind of plates you need to order and use for our experiments?
*Lamp energy and Noise signal ratios??


<br clear="all">
Talk about processing data
 
*Export format?
desc | file 14:20, 10 July 2007 PKU_Switch_07-7-10_RD2.gif Chen Daizhuo 7,605 (l to r: RD2(1-5), ladder)
*How to export data from fluorometer?
desc | file 14:07, 10 July 2007 ECFP-pCC002.gif Chen Daizhuo 12,705 (l to r: Marker, ECFP-pCC002(1-15), control)
*Built-in data analysis tools?
desc | file 03:58, 10 July 2007 Mutagenesisgel.jpg AlexanderPickett 135,807 
*Technical Support.
desc | file 22:36, 9 July 2007 Kemp_Labmembers_JW.jpg Mlambeth 1,537,795 
desc | file 22:34, 9 July 2007 Higgs-headshot.jpg Gadareth Higgs 23,161 
desc | file 21:40, 9 July 2007 Moratorium.doc Adamsr 37,888 
desc | file 21:39, 9 July 2007 SynBERC.doc Adamsr 25,088
 
[[MEDIA:otherfiles.ppt|blablabla]]

Revision as of 08:03, 1 August 2007

Questions to ask the fluorometer Engineer on Friday, Aug 3

See if our plate reader has the ability to measure what you want to measure.

  • What are the classes of measurement? Can it do time-resolved fluorometry + Absorbance?
  • Delay time-resolve - 24 hour experiment; evaporation of solution over long-time courses?!
  • Temperature-regulation?
  • How do we subtract background values from data and convert from absorbance to OD600?

Talk about using the plate reader and creating protocols.

  • Excitation filters and Emission filters for GFP, acGFP and DsRed-Express?
  • Any manufacturer protocols for plate reader usage?
  • Well plate rows and columns? How is data arranged sequentially with the wells?
  • What kind of plates you need to order and use for our experiments?
  • Lamp energy and Noise signal ratios??

Talk about processing data

  • Export format?
  • How to export data from fluorometer?
  • Built-in data analysis tools?
  • Technical Support.
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