User:Alexander.wong/sandbox

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(Questions to ask the fluorometer Engineer on Friday, Aug 3)
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*Built-in data analysis tools?
*Built-in data analysis tools?
*Technical Support.
*Technical Support.
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==Experiment 1: Fluorescence vs extracellular [GFP] molecules==
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Known concentrations of GFP are diluted into a range of dilutions and are then mixed with cell lysate of a particular chassis.
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===Materials Required===
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====Equipment====
 +
 +
 +
====Reagents====
 +
 +
===Protocol===
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 +
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==Experiment 2: Fluorescence vs Cultures of varying unknown intracellular [GFP]==
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Measuring the fluorescence of cultures of cells at set time intervals would measure a set of unknown intracellular [GFP].
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===Materials Required===
 +
====Equipment====
 +
 +
 +
 +
====Reagents====
 +
 +
===Protocol===
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 +
 +
 +
==Experiment 3: Fluorescence vs Cultures of varying unknown extracellular [GFP]==
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Lysing samples of Experiment 2 will allow the relation of an unknwon intracellular [GFP] to that of extracellular [GFP].
 +
===Materials Required===
 +
====Equipment====
 +
 +
 +
 +
====Reagents====
 +
 +
===Protocol===

Revision as of 06:25, 2 August 2007

Contents

Questions to ask the fluorometer Engineer on Friday, Aug 3

See if our plate reader has the ability to measure what you want to measure.

  • What are the classes of measurement? Can it do time-resolved fluorometry + Absorbance?
  • Delay time-resolve - 24 hour experiment; evaporation of solution over long-time courses?!
  • Temperature-regulation?
  • How do we subtract background values from data and convert from absorbance to OD600?

Talk about using the plate reader and creating protocols.

  • Excitation filters and Emission filters for GFP, acGFP and DsRed-Express?
  • Any manufacturer protocols for plate reader usage?
  • Well plate rows and columns? How is data arranged sequentially with the wells?
  • What kind of plates you need to order and use for our experiments?
  • Lamp energy and Noise signal ratios??

Talk about processing data

  • Export format?
  • How to export data from fluorometer?
  • Built-in data analysis tools?
  • Technical Support.


Experiment 1: Fluorescence vs extracellular [GFP] molecules

Known concentrations of GFP are diluted into a range of dilutions and are then mixed with cell lysate of a particular chassis.

Materials Required

Equipment

Reagents

Protocol

Experiment 2: Fluorescence vs Cultures of varying unknown intracellular [GFP]

Measuring the fluorescence of cultures of cells at set time intervals would measure a set of unknown intracellular [GFP].

Materials Required

Equipment

Reagents

Protocol

Experiment 3: Fluorescence vs Cultures of varying unknown extracellular [GFP]

Lysing samples of Experiment 2 will allow the relation of an unknwon intracellular [GFP] to that of extracellular [GFP].

Materials Required

Equipment

Reagents

Protocol

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