User:Alexander.wong/sandbox

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(New page: Picture This is a picture desc | file 15:00, 12 July 2007 ICGEMS_board1.jpg Dirk Van Swaay 63,548 (testing file) desc | file 14:32, 12 July 2007 Lux_o...)
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[[ IMAGE: ICGEMS_board1.jpg|frame|Picture]] This is a picture
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=Layout for New iGEM page=
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<div id="mainpage">
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<!-- Heading -->
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<hr class=divider>
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{| style="width:800px; height:80px;"
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|-
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| width="220px" rowspan=1 | <center><html><a href="http://www.imperial.ac.uk"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/281904.GIF" height="55"></a></html></center>
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| width="390px" |  <br><center><html><h3>THE IMPERIAL iGEM 2007 Team </h3></html></center>
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| width="110px"| <html><a href="http://www.imperial.ac.uk/centenary"><p align="right"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/7813700.GIF" height="60"></p></a></html>
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|- valign="center"
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| {{Click || image=Protocols red.png | link=IGEM:IMPERIAL/2007/Wet Lab| width=40px | height=40px }}
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| [[IGEM:IMPERIAL/2007/Wet Lab|<font face="Arial" size=3 style="color:#000099">'''In the Wet Lab'''</font>]]<br /><font size=1>lab notebook, protocols</font>
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|- valign="center"
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| {{Click || image=Labs grey.png | link=IGEM:IMPERIAL/2007/Dry Lab| width=40px | height=40px }}
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| [[IGEM:IMPERIAL/2007/Dry Lab|<font face="Arial" size=3 style="color:#000099">'''In the Dry Lab'''</font>]]<br /><font size=1>modelling, data analysis</font>
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<br>
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|- valign="center"
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| {{Click || image=Courses violet.png | link=IGEM:IMPERIAL/2007/Resources| width=40px | height=40px }}
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| [[IGEM:IMPERIAL/2007/Resources|<font face="Arial" size=3 style="color:#000099">'''Resources'''</font>]]<br /><font size=1>calendar, literature, links</font>
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<br>
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|- valign="center"
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| {{Click || image=About blue.png | link=IGEM:IMPERIAL/2007/Meet The Team | width=40px | height=40px }}
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| [[IGEM:IMPERIAL/2007/Meet The Team|<font face="Arial" size=3 style="color:#000099">'''Meet the Team'''</font>]]<br/><font size=1>learn more about us</font>
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|- valign="center"
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| {{Click || image=Gettingstarted green.png | link=IGEM:IMPERIAL/2007/Entertainment| width=40px | height=40px }}
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| [[IGEM:IMPERIAL/2007/Entertainment|<font face="Arial" size=3 style="color:#000099">'''Entertainment'''</font>]]<br /><font size=1>pics, quotes, the logo</font>
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<br>
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|- valign="center"
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| {{Click || image=Groups turquoise.png | link=IGEM:IMPERIAL/2007/Feedback| width=40px | height=40px }}
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| [[IGEM:IMPERIAL/2007/Feedback|<font face="Arial" size=3 style="color:#000099">'''Contact Us'''</font>]]<br /><font size=1>we are feedback friendly</font>
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'''In partnership with'''
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<html><a href="http://www.geneart.com"><img src="http://www.geneart.com/fileadmin/templates/img/logo.gif" width="200"></a></html>
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<a href="http://www3.clustrmaps.com/counter/maps.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" /></a></center></html>
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desc | file 15:00, 12 July 2007 ICGEMS_board1.jpg Dirk Van Swaay 63,548 (testing file)
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<br><br>
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desc | file 14:32, 12 July 2007 Lux_overnight_fluorescence_7_11_07.xls Guido 78,848 
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desc | file 13:24, 12 July 2007 PKU_Switch_07-7-12_SDD_EGFP1-12_RD1_13-18_CD1_19-23.jpg Chen Daizhuo 6,338 (l to r: SDD_EGFP_pCC002(1-12), RD1(13-18), CD1(19-23), pMDB, ladder)
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desc | file 13:19, 12 July 2007 PKU_Switch_07-7-12_SDD'_EGFP-pCC002_digestion_again.jpg Chen Daizhuo 9,992 (l to r: Marker, SDD'_EGFP_pCC002(1-8))
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desc | file 06:33, 12 July 2007 07.07.11_-colony.JPG Nishant M. Bhat 51,714 
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desc | file 06:28, 12 July 2007 7-7-11-e0240bb.JPG Nishant M. Bhat 61,979 
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desc | file 05:40, 12 July 2007 7-7-10.jpg Nishant M. Bhat 57,748 
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desc | file 00:49, 12 July 2007 Example_of_an_empty_file.txt Ben Yi Tew 63 
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desc | file 00:37, 12 July 2007 My_adorable_sheepy.gif Ben Yi Tew 3,418 
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desc | file 23:37, 11 July 2007 My_adorable_sheepy.png Ben Yi Tew 830 (Just a very adorable sheep...)
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desc | file 23:07, 11 July 2007 Header1.png Kun Zhang 40,682 
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desc | file 20:31, 11 July 2007 07.07.10.jpg Jessica Karen Wong 31,601 
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desc | file 20:30, 11 July 2007 2007-07-11_perryColonyPCRgx.jpg GeorgeXu 121,839 
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desc | file 19:37, 11 July 2007 I_feed_large.gif Abhishektiwari 1,737 
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desc | file 19:03, 11 July 2007 Tripwire.gif Ilya 4,679 
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desc | file 18:16, 11 July 2007 Tab_icon.png Ben Yi Tew 1,135 
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desc | file 15:39, 11 July 2007 PKU_bear_logo.gif Chen Daizhuo 49,078 (by CXY~)
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desc | file 15:30, 11 July 2007 PKU_Switch_07-7-11_SD1-3_ECFP_pCC002.gif Chen Daizhuo 4,937 
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desc | file 15:05, 11 July 2007 PKU_Switch_07-7-11_SDD'-EGFP-pCC002_SDD'-EGFP-BamHI-F&pCCtestR.gif Chen Daizhuo 9,981 (SDD'-EGFP-pCC002(13-35), Negative Control, Marker)
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desc | file 15:03, 11 July 2007 SS3_SS2_SDD'-EGFP-pCC002_pcr_test.gif Chen Daizhuo 10,793 (l to r: SS3(31-32), SS2(37-44), SDD'-EGFP-pCC002(1-12), none, Negative control for SS2, Marker)
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desc | file 15:00, 11 July 2007 SS1_SD3_pcr_test_larger.gif Chen Daizhuo 14,000 (l to r: Negative control, SS1(1-10), SD3(13-18), SS3(25-30), RD1, Marker)
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desc | file 14:14, 11 July 2007 Macintosh_HD-Users-nkuldell-Desktop-Enzymes_in_20109lab.doc Nkuldell 19,968 
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desc | file 05:51, 11 July 2007 HBNG.pdf Abhishektiwari 183,129 
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desc | file 02:38, 11 July 2007 11-07-07_1733.jpg Lucas A. Hadjilucas 42,439 (Test image)
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desc | file 20:55, 10 July 2007 Colonypcrpt071007.jpg Shlo 153,008 
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desc | file 20:30, 10 July 2007 SB_Primer_100707.pdf Scmohr 10,278,992 (First posted draft (7/10/07))
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desc | file 20:26, 10 July 2007 Colonypcrgx071007.jpg Shlo 171,241 
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desc | file 20:22, 10 July 2007 Andrew_hessel.JPG Ahessel 41,270 
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desc | file 20:14, 10 July 2007 Colonypcrsl0710073min.jpg Shlo 156,834 
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desc | file 18:46, 10 July 2007 ThumbWiki.png KMagic246 39,027 
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desc | file 18:39, 10 July 2007 Webpage.png KMagic246 106,781 
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<!-- Links to be deleted -->
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'''Useful Links'''
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<br clear="all">
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* '''[[IGEM:IMPERIAL/2007/Sandbox|Original Layout]]'''
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* '''[[IGEM:IMPERIAL/2007/Projects/Experimental Design | Experimental Design]]'''
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* '''[[IGEM:IMPERIAL/2007/Projects/In-Veso|In-Veso Gene Expression]]'''
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|width="560px" class="white" valign="top"|
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<!-- Main page-->
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=== Our Projects ===
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<br>
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{|class="white" align="center" cellpadding="0"
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|- valign="center"
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| {{Click || image=Service_lab.png | link=IGEM:IMPERIAL/2007/Projects/Cell By Date | width=40px | height=40px }}
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| [[IGEM:IMPERIAL/2007/Projects/Cell By Date|<font face="Arial" size=3 style="color:#000099">'''Cell by Date'''</font>]]<br /><font size=2>Thermal Bioindicator</font>
 +
| {{Click || image=Service_lab.png | link=IGEM:IMPERIAL/2007/Biofilm Detector | width=40px | height=40px }}
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| [[IGEM:IMPERIAL/2007/Biofilm Detector|<font face="Arial" size=3 style="color:#000099">'''Infector Detector'''</font>]]<br /><font size=2>Preventing MRSA</font>
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|}
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[[image:IC07_CFS_cartoon.png |center| 320px]]
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{|class="white" align="center" cellpadding="0"
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|- valign="center"
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| {{Click || image=Resources yellow.png | link=IGEM:IMPERIAL/2007/CFS| width=40px | height=40px }}
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| [[IGEM:IMPERIAL/2007/CFS|<font face="Arial" size=4 style="color:#000099">'''Cell Free Systems'''</font>]]<br /><font size=3>''Dead Sexy!''</font>
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<br>
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|}
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<hr />
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<!-- Breaking News -->
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{|class="white" align="left" cellpadding="0"
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|- valign="center"
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| {{Click || image=Community orange.png | link=OpenWetWare:Community Portal| width=40px | height=40px }}
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| [[OpenWetWare:Community Portal|<font face="Arial" size=2 style="color:#ee0000">'''Breaking News'''</font>]]<br /><font size=1><html><marquee direction="up" height="20" width="400" scrolldelay="300" scrollamount="1">
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<br>
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<b><font style="color:red">28/08/07</font> - </b>Infector Detector Modelling!! <br>
 +
<br>
 +
<b><font style="color:red">15/08/07</font> - </b>We have vesicles!! <br>
 +
<br><br>
 +
</marquee></html></font>
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|}
<br clear="all">
<br clear="all">
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<font size="3"><center>'''28 August 2007 - Infector Modelling'''</center></font>
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[[Image:ICGEMS ID tf C2 3D1(mesh-grid).jpg|thumb|center|280px|Modelled [[IGEM:IMPERIAL/2007/Projects/Biofilm_Detector/Modelling|transfer function]] of Infector Detector. Not only has it aesthetic qualities - it actually is very informative!]]
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<hr />
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<!-- Recent Changes -->
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<h3><html>
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<img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px">
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</html>Recent Changes</h3>
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{{Special:Recentchanges/b=IGEM:IMPERIAL/2007&limit=10}}
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= Layout for new wiki v.2 =
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<div id="mainpage">
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{| style="width:800px; height:70px;"
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| width="160px" rowspan=1 | <html><a href="http://www.imperial.ac.uk"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/281904.GIF" height="55"></a></html>
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| width="160px"| <html><a href="http://www.imperial.ac.uk/centenary"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/7813700.GIF" height="60"></a></html>
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<hr class=divider>
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{| cellspacing="5"
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|width="200px" class="blue" valign="top"|
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{|class="blue" align="center" cellpadding="0"
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|- valign="center"
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| {{Click || image=Courses violet.png | link=Courses| width=40px | height=40px }}
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| [[Courses|<font face="Arial" size=3 style="color:#000099">'''Resources'''</font>]]<br /><font size=1>calendar, literature, links</font>
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|- valign="center"
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| {{Click || image=Protocols red.png | link=Protocols| width=40px | height=40px }}
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| [[Protocols|<font face="Arial" size=3 style="color:#000099">'''In the Wet Lab'''</font>]]<br /><font size=1>lab notebook, protocols</font>
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|- valign="center"
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| {{Click || image=Labs grey.png | link=Labs| width=40px | height=40px }}
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| [[Labs|<font face="Arial" size=3 style="color:#000099">'''Modelling'''</font>]]<br /><font size=1>approach, data analysis</font>
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<br>
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|- valign="center"
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| {{Click || image=About blue.png | link=OpenWetWare:About | width=40px | height=40px }}
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| [[OpenWetWare:About|<font face="Arial" size=3 style="color:#000099">'''Meet the Team'''</font>]]<br/><font size=1>learn more about us</font>
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|- valign="center"
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| {{Click || image=Gettingstarted green.png | link=Help:Contents | width=40px | height=40px }}
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| [[Help:Contents|<font face="Arial" size=3 style="color:#000099">'''Entertainment'''</font>]]<br /><font size=1>pics, quotes, the logo</font>
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|}
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|width="400px" class="white" valign="top"|
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<!-- INFORMATION HEADER with edit link-->
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{|class="white" align="center" cellpadding="0"
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|- valign="center"
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| {{Click || image=Resources yellow.png | link=IGEM:IMPERIAL/2007/Projects/In-Veso| width=40px | height=40px }}
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| [[IGEM:IMPERIAL/2007/Projects/In-Veso|<font face="Arial" size=4 style="color:#000099">'''Cell Free Systems'''</font>]]<br /><font size=2>''The Future of Synthetic Biology?''</font>
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|}
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[[image:IC07_CFS_cartoon.png |center| 280px]]
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{|class="white" align="center" cellpadding="0"
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|- valign="center"
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| {{Click || image=Service_lab.png | link=IGEM:IMPERIAL/2007/Projects/Cell By Date | width=40px | height=40px }}
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| [[IGEM:IMPERIAL/2007/Projects/Cell By Date|<font face="Arial" size=3 style="color:#000099">'''Cell by Date'''</font>]]<br /><font size=2>Thermal Bioindicator</font>
 +
| {{Click || image=Service_lab.png | link=IGEM:IMPERIAL/2007/Biofilm Detector | width=40px | height=40px }}
 +
| [[IGEM:IMPERIAL/2007/Biofilm Detector|<font face="Arial" size=3 style="color:#000099">'''Infector Detector'''</font>]]<br /><font size=2>Preventing MRSA</font>
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|}
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|width="200px" class="blue" valign="top"|
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{|class="blue" align="center" cellpadding="0"
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|- valign="left"
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| {{Click || image=Community orange.png | link=OpenWetWare:Community Portal| width=40px | height=40px }}
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| [[OpenWetWare:Community Portal|<font face="Arial" size=3 style="color:#000099">'''News Flash'''</font>]]<br /><font size=1>the highlights</font>
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|}
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<center><html><marquee direction="up" height="30" width="160" scrolldelay="300" scrollamount="2">
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<br>
 +
<b><font style="color:red">28/08/07</font> - </b>Infector Detector Modelling!! <br>
 +
<br>
 +
<b><font style="color:red">15/08/07</font> - </b>We have vesicles!! <br>
 +
<br><br>
 +
</marquee></html></center>
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<br>
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{|class="blue" align="center" cellpadding="0"
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|- valign="center"
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| [[OpenWetWare:Community Portal|<font face="Arial" size=3 style="color:#000099">'''Our Visitors'''</font>]]<br /><font size=1>feedback welcome</font>
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</a></html></center>
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|}
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<hr class=divider>
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<br clear="all">
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'''In partnership with'''
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<br clear="all">
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<html><a href="http://www.geneart.com"><img src="http://www.geneart.com/fileadmin/templates/img/logo.gif" width="200"></a></html>
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<br>
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{| cellspacing="5"
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<h3><html>
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<img src="http://www.openwetware.org/images/c/c1/RecentChanges_icon.png" width="20px">
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</html>Recent Changes</h3>
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<hr/>{{Special:Recentchanges/b=IGEM:IMPERIAL/2007&limit=10}}
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|}
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</div>
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<!-- BLA BLA BLA--!>
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| colspan="2" | <html>
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<a href="http://www3.clustrmaps.com/counter/maps.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=http://www.openwetware.com/wiki/IGEM:IMPERIAL/2007" width="160" />
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</a></html>
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|}
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{|class="white" cellpadding="0"
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|-
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| <font size=2>'''Breaking News :'''</font>
 +
| <html><marquee direction="up" height="20"  scrolldelay="300" scrollamount="1">
 +
<br>
 +
<b>28/08/07 - </b>Infector Detector Modelling!! <br>
 +
<br>
 +
<b>15/08/07 - </b>We have vesicles!! <br>
 +
<br><br>
 +
</marquee></html>
 +
|}
 +
 +
 +
 +
 +
<center>
 +
<big>'''[[IGEM:IMPERIAL/2007/Projects/In-Veso|Cell Free Systems]]'''</big>
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[[Image:IC07_CFS_cartoon.png|350px]]</center>
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<br>
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{|border="0" width="100%" align="center"
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! <big><html>
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<img src="http://www.openwetware.org/images/8/84/Service_lab.png" alt="INFORMATION" border="0" width="40px">
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</html>'''[[IGEM:IMPERIAL/2007/Projects/Cell By Date|Cell By Date]] '''</big> !! <big><html>
 +
<img src="http://www.openwetware.org/images/8/84/Service_lab.png" alt="INFORMATION" border="0" width="40px">
 +
</html>'''[[IGEM:IMPERIAL/2007/Biofilm Detector|Infector Detector]]'''</big>
 +
|}<br>
 +
 +
{|class="white" align="center" width="95%" cellpadding="0"
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|- valign="center"
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| {{Click || image=About blue.png | link=OpenWetWare:About | width=40px | height=40px }}
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| [[OpenWetWare:About|<font face="Arial" size=2 style="color:#000099">'''About Us'''</font>]]<br/><font size=1>Learn more about us</font>
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| {{Click || image=Gettingstarted green.png | link=Help:Contents | width=40px | height=40px }}
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| [[Help:Contents|<font face="Arial" size=2 style="color:#000099">'''Help'''</font>]]<br /><font size=1>Step-by-step tutorials</font>
 +
| {{Click || image=Resources yellow.png | link=Resources| width=40px | height=40px }}
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| [[Resources|<font face="Arial" size=2 style="color:#000099">'''Resources'''</font>]]<br /><font size=1>Useful links & tools</font>
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| {{Click || image=Community orange.png | link=OpenWetWare:Community Portal| width=40px | height=40px }}
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| [[OpenWetWare:Community Portal|<font face="Arial" size=2 style="color:#000099">'''Community'''</font>]]<br /><font size=1>How you can help</font>
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|- valign="center"
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| {{Click || image=Labs grey.png | link=Labs| width=40px | height=40px }}
 +
| [[Labs|<font face="Arial" size=2 style="color:#000099">'''Labs'''</font>]]<br /><font size=1>From around the world</font>
 +
| {{Click || image=Protocols red.png | link=Protocols| width=40px | height=40px }}
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| [[Protocols|<font face="Arial" size=2 style="color:#000099">'''Protocols'''</font>]]<br /><font size=1>Share techniques & more</font>
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| {{Click || image=Courses violet.png | link=Courses| width=40px | height=40px }}
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| [[Courses|<font face="Arial" size=2 style="color:#000099">'''Courses'''</font>]]<br /><font size=1>Host & view classes</font>
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| {{Click || image=Groups turquoise.png | link=Groups| width=40px | height=40px }}
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| [[Groups|<font face="Arial" size=2 style="color:#000099">'''Groups'''</font>]]<br /><font size=1>Host & view organizations</font>
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|}
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<hr>
 +
 +
==Questions to ask the fluorometer Engineer on Friday, Aug 3==
 +
 +
See if our plate reader has the ability to measure what you want to measure.
 +
*What are the classes of measurement? Can it do time-resolved fluorometry + Absorbance?
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*Delay time-resolve - 24 hour experiment; evaporation of solution over long-time courses?!
 +
*Temperature-regulation?
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* How do we subtract background values from data and convert from absorbance to OD600?
 +
 +
Talk about using the plate reader and creating protocols.
 +
*Excitation filters and Emission filters for GFP, acGFP and DsRed-Express?
 +
*Any manufacturer protocols for plate reader usage?
 +
*Well plate rows and columns? How is data arranged sequentially with the wells?
 +
*What kind of plates you need to order and use for our experiments?
 +
*Lamp energy and Noise signal ratios??
 +
 +
Talk about processing data
 +
*Export format?
 +
*How to export data from fluorometer?
 +
*Built-in data analysis tools?
 +
*Technical Support.
 +
 +
 +
==Experiment 1: Fluorescence vs extracellular [GFP] molecules==
 +
Known concentrations of GFP are diluted into a range of dilutions and are then mixed with cell lysate of a particular chassis.
 +
===Materials Required===
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====Equipment====
 +
 +
 +
====Reagents====
 +
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===Protocol===
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 +
 +
==Experiment 2: Fluorescence vs Cultures of varying unknown intracellular [GFP]==
 +
Measuring the fluorescence of cultures of cells at set time intervals would measure a set of unknown intracellular [GFP].
 +
===Materials Required===
 +
====Equipment====
 +
 +
 +
 +
====Reagents====
 +
 +
===Protocol===
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 +
 +
 +
==Experiment 3: Fluorescence vs Cultures of varying unknown extracellular [GFP]==
 +
Lysing samples of Experiment 2 will allow the relation of an unknwon intracellular [GFP] to that of extracellular [GFP].
 +
===Materials Required===
 +
====Equipment====
 +
 +
-
desc | file 14:20, 10 July 2007 PKU_Switch_07-7-10_RD2.gif Chen Daizhuo 7,605 (l to r: RD2(1-5), ladder)
+
====Reagents====
-
desc | file 14:07, 10 July 2007 ECFP-pCC002.gif Chen Daizhuo 12,705 (l to r: Marker, ECFP-pCC002(1-15), control)
+
-
desc | file 03:58, 10 July 2007 Mutagenesisgel.jpg AlexanderPickett 135,807 
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-
desc | file 22:36, 9 July 2007 Kemp_Labmembers_JW.jpg Mlambeth 1,537,795 
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desc | file 22:34, 9 July 2007 Higgs-headshot.jpg Gadareth Higgs 23,161 
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desc | file 21:40, 9 July 2007 Moratorium.doc Adamsr 37,888 
+
-
desc | file 21:39, 9 July 2007 SynBERC.doc Adamsr 25,088
+
-
[[MEDIA:otherfiles.ppt|blablabla]]
+
===Protocol===

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28 August 2007 - Infector Modelling
Modelled transfer function of Infector Detector. Not only has it aesthetic qualities - it actually is very informative!
Modelled transfer function of Infector Detector. Not only has it aesthetic qualities - it actually is very informative!

Recent Changes






Layout for new wiki v.2


Resources
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In the Wet Lab
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Modelling
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Meet the Team
learn more about us
Entertainment
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Cell Free Systems
The Future of Synthetic Biology?
Cell by Date
Thermal Bioindicator
Infector Detector
Preventing MRSA
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28/08/07 - Infector Detector Modelling!!

15/08/07 - We have vesicles!!



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