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[[ IMAGE: ICGEMS_board1.jpg|frame|Picture]] This is a picture
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| width="390px" |  <br><center><html><h3>THE IMPERIAL iGEM 2007 Team </h3></html></center>
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desc | file 15:00, 12 July 2007 ICGEMS_board1.jpg Dirk Van Swaay 63,548 (testing file)
desc | file 14:32, 12 July 2007 Lux_overnight_fluorescence_7_11_07.xls Guido 78,848 
desc | file 13:24, 12 July 2007 PKU_Switch_07-7-12_SDD_EGFP1-12_RD1_13-18_CD1_19-23.jpg Chen Daizhuo 6,338 (l to r: SDD_EGFP_pCC002(1-12), RD1(13-18), CD1(19-23), pMDB, ladder)
desc | file 13:19, 12 July 2007 PKU_Switch_07-7-12_SDD'_EGFP-pCC002_digestion_again.jpg Chen Daizhuo 9,992 (l to r: Marker, SDD'_EGFP_pCC002(1-8))
desc | file 06:33, 12 July 2007 07.07.11_-colony.JPG Nishant M. Bhat 51,714 
desc | file 06:28, 12 July 2007 7-7-11-e0240bb.JPG Nishant M. Bhat 61,979 
desc | file 05:40, 12 July 2007 7-7-10.jpg Nishant M. Bhat 57,748 
desc | file 00:49, 12 July 2007 Example_of_an_empty_file.txt Ben Yi Tew 63 
desc | file 00:37, 12 July 2007 My_adorable_sheepy.gif Ben Yi Tew 3,418 
desc | file 23:37, 11 July 2007 My_adorable_sheepy.png Ben Yi Tew 830 (Just a very adorable sheep...)
desc | file 23:07, 11 July 2007 Header1.png Kun Zhang 40,682 
desc | file 20:31, 11 July 2007 07.07.10.jpg Jessica Karen Wong 31,601 
desc | file 20:30, 11 July 2007 2007-07-11_perryColonyPCRgx.jpg GeorgeXu 121,839 
desc | file 19:37, 11 July 2007 I_feed_large.gif Abhishektiwari 1,737 
desc | file 19:03, 11 July 2007 Tripwire.gif Ilya 4,679 
desc | file 18:16, 11 July 2007 Tab_icon.png Ben Yi Tew 1,135 
desc | file 15:39, 11 July 2007 PKU_bear_logo.gif Chen Daizhuo 49,078 (by CXY~)
desc | file 15:30, 11 July 2007 PKU_Switch_07-7-11_SD1-3_ECFP_pCC002.gif Chen Daizhuo 4,937 
desc | file 15:05, 11 July 2007 PKU_Switch_07-7-11_SDD'-EGFP-pCC002_SDD'-EGFP-BamHI-F&pCCtestR.gif Chen Daizhuo 9,981 (SDD'-EGFP-pCC002(13-35), Negative Control, Marker)
desc | file 15:03, 11 July 2007 SS3_SS2_SDD'-EGFP-pCC002_pcr_test.gif Chen Daizhuo 10,793 (l to r: SS3(31-32), SS2(37-44), SDD'-EGFP-pCC002(1-12), none, Negative control for SS2, Marker)
desc | file 15:00, 11 July 2007 SS1_SD3_pcr_test_larger.gif Chen Daizhuo 14,000 (l to r: Negative control, SS1(1-10), SD3(13-18), SS3(25-30), RD1, Marker)
desc | file 14:14, 11 July 2007 Macintosh_HD-Users-nkuldell-Desktop-Enzymes_in_20109lab.doc Nkuldell 19,968 
desc | file 05:51, 11 July 2007 HBNG.pdf Abhishektiwari 183,129 
desc | file 02:38, 11 July 2007 11-07-07_1733.jpg Lucas A. Hadjilucas 42,439 (Test image)
desc | file 20:55, 10 July 2007 Colonypcrpt071007.jpg Shlo 153,008 
desc | file 20:30, 10 July 2007 SB_Primer_100707.pdf Scmohr 10,278,992 (First posted draft (7/10/07))
desc | file 20:26, 10 July 2007 Colonypcrgx071007.jpg Shlo 171,241 
desc | file 20:22, 10 July 2007 Andrew_hessel.JPG Ahessel 41,270 
desc | file 20:14, 10 July 2007 Colonypcrsl0710073min.jpg Shlo 156,834 
desc | file 18:46, 10 July 2007 ThumbWiki.png KMagic246 39,027 
desc | file 18:39, 10 July 2007 Webpage.png KMagic246 106,781 




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<!-- Main page-->
=== Our Projects ===
<br>
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| [[IGEM:IMPERIAL/2007/Projects/Cell By Date|<font face="Arial" size=3 style="color:#000099">'''Cell by Date'''</font>]]<br /><font size=2>Thermal Bioindicator</font>
| {{Click || image=Service_lab.png | link=IGEM:IMPERIAL/2007/Biofilm Detector | width=40px | height=40px }}
| [[IGEM:IMPERIAL/2007/Biofilm Detector|<font face="Arial" size=3 style="color:#000099">'''Infector Detector'''</font>]]<br /><font size=2>Preventing MRSA</font>
|}
[[image:IC07_CFS_cartoon.png |center| 320px]]
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<b><font style="color:red">28/08/07</font> - </b>Infector Detector Modelling!! <br>
<br>
<b><font style="color:red">15/08/07</font> - </b>We have vesicles!! <br>
<br><br>
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<font size="3"><center>'''28 August 2007 - Infector Modelling'''</center></font>
[[Image:ICGEMS ID tf C2 3D1(mesh-grid).jpg|thumb|center|280px|Modelled [[IGEM:IMPERIAL/2007/Projects/Biofilm_Detector/Modelling|transfer function]] of Infector Detector. Not only has it aesthetic qualities - it actually is very informative!]]
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<b><font style="color:red">28/08/07</font> - </b>Infector Detector Modelling!! <br>
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<b><font style="color:red">15/08/07</font> - </b>We have vesicles!! <br>
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<br><br>
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</html>'''[[IGEM:IMPERIAL/2007/Projects/Cell By Date|Cell By Date]] '''</big> !! <big><html>
<img src="http://www.openwetware.org/images/8/84/Service_lab.png" alt="INFORMATION" border="0" width="40px">
</html>'''[[IGEM:IMPERIAL/2007/Biofilm Detector|Infector Detector]]'''</big>
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<hr>
==Questions to ask the fluorometer Engineer on Friday, Aug 3==
See if our plate reader has the ability to measure what you want to measure.
*What are the classes of measurement? Can it do time-resolved fluorometry + Absorbance?
*Delay time-resolve - 24 hour experiment; evaporation of solution over long-time courses?!
*Temperature-regulation?
* How do we subtract background values from data and convert from absorbance to OD600?
Talk about using the plate reader and creating protocols.
*Excitation filters and Emission filters for GFP, acGFP and DsRed-Express?
*Any manufacturer protocols for plate reader usage?
*Well plate rows and columns? How is data arranged sequentially with the wells?
*What kind of plates you need to order and use for our experiments?
*Lamp energy and Noise signal ratios??
Talk about processing data
*Export format?
*How to export data from fluorometer?
*Built-in data analysis tools?
*Technical Support.
==Experiment 1: Fluorescence vs extracellular [GFP] molecules==
Known concentrations of GFP are diluted into a range of dilutions and are then mixed with cell lysate of a particular chassis.
===Materials Required===
====Equipment====
====Reagents====
===Protocol===
==Experiment 2: Fluorescence vs Cultures of varying unknown intracellular [GFP]==
Measuring the fluorescence of cultures of cells at set time intervals would measure a set of unknown intracellular [GFP].
===Materials Required===
====Equipment====
====Reagents====
===Protocol===
==Experiment 3: Fluorescence vs Cultures of varying unknown extracellular [GFP]==
Lysing samples of Experiment 2 will allow the relation of an unknwon intracellular [GFP] to that of extracellular [GFP].
===Materials Required===
====Equipment====


desc | file 14:20, 10 July 2007 PKU_Switch_07-7-10_RD2.gif Chen Daizhuo 7,605 (l to r: RD2(1-5), ladder)
====Reagents====
desc | file 14:07, 10 July 2007 ECFP-pCC002.gif Chen Daizhuo 12,705 (l to r: Marker, ECFP-pCC002(1-15), control)
desc | file 03:58, 10 July 2007 Mutagenesisgel.jpg AlexanderPickett 135,807 
desc | file 22:36, 9 July 2007 Kemp_Labmembers_JW.jpg Mlambeth 1,537,795 
desc | file 22:34, 9 July 2007 Higgs-headshot.jpg Gadareth Higgs 23,161 
desc | file 21:40, 9 July 2007 Moratorium.doc Adamsr 37,888 
desc | file 21:39, 9 July 2007 SynBERC.doc Adamsr 25,088


[[MEDIA:otherfiles.ppt|blablabla]]
===Protocol===

Latest revision as of 00:29, 11 September 2007

Layout for New iGEM page


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<html><h3>THE IMPERIAL iGEM 2007 Team </h3></html>
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Thermal Bioindicator
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<br> <b><font style="color:red">28/08/07</font> - </b>Infector Detector Modelling!! <br> <br> <b><font style="color:red">15/08/07</font> - </b>We have vesicles!! <br> <br><br> </marquee></html>


28 August 2007 - Infector Modelling
Modelled transfer function of Infector Detector. Not only has it aesthetic qualities - it actually is very informative!

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25 April 2024

     00:22  The paper that launched microfluidics - Xi Ning‎‎ 7 changes history +4,723 [Xning098‎ (7×)]
     
00:22 (cur | prev) −97 Xning098 talk contribs
     
00:18 (cur | prev) +14 Xning098 talk contribs (→‎Summary)
     
00:11 (cur | prev) +1 Xning098 talk contribs (→‎Introduction)
     
00:11 (cur | prev) +1 Xning098 talk contribs (→‎Introduction)
     
00:11 (cur | prev) 0 Xning098 talk contribs (→‎References)
     
00:08 (cur | prev) +4 Xning098 talk contribs (→‎Significance)
     
00:07 (cur | prev) +4,800 Xning098 talk contribs

24 April 2024

     22:50  WAKNA:Basics‎‎ 8 changes history +610 [Berthold Drexler‎ (8×)]
     
22:50 (cur | prev) +136 Berthold Drexler talk contribs (→‎Hier finden Sie Literatur für Einsteiger:innen in das Gebiet der Neuroanästhesie)
     
22:47 (cur | prev) +238 Berthold Drexler talk contribs (→‎Neuromonitoring allgemein)
     
22:33 (cur | prev) +151 Berthold Drexler talk contribs (→‎Hier finden Sie Literatur für Einsteiger:innen in das Gebiet der Neuroanästhesie)
     
22:31 (cur | prev) 0 Berthold Drexler talk contribs (→‎Grundlagen, Physiologie & Pharmakologie)
     
22:30 (cur | prev) +1 Berthold Drexler talk contribs (→‎Grundlagen, Physiologie & Pharmakologie)
     
22:30 (cur | prev) +313 Berthold Drexler talk contribs (→‎Grundlagen, Physiologie & Pharmakologie)
     
06:23 (cur | prev) +192 Berthold Drexler talk contribs (→‎Hier finden Sie Literatur für Einsteiger:innen in das Gebiet der Neuroanästhesie)
     
06:21 (cur | prev) −421 Berthold Drexler talk contribs (→‎Sonstige)
     18:35  User:Yanbin Huang‎‎ 2 changes history +25 [Yanbin Huang‎ (2×)]
     
18:35 (cur | prev) +13 Yanbin Huang talk contribs (→‎Peer-Reviewed Publications)
     
18:34 (cur | prev) +12 Yanbin Huang talk contribs (→‎Peer-Reviewed Publications)
     17:49  Hu‎‎ 2 changes history +28 [Hugangqing‎ (2×)]
     
17:49 (cur | prev) +18 Hugangqing talk contribs
     
17:48 (cur | prev) +10 Hugangqing talk contribs
     08:14  "Pick and Place" Assembly of Parts Using PDMS - Amy Lim, Rylie Costello‎‎ 6 changes history +394 [Rcostello‎ (6×)]
     
08:14 (cur | prev) +1 Rcostello talk contribs (→‎"Pick and Place" Methodology)
     
08:13 (cur | prev) −14 Rcostello talk contribs (→‎"Pick and Place" Methodology)
     
08:12 (cur | prev) −1 Rcostello talk contribs (→‎"Pick and Place" Methodology)
     
08:12 (cur | prev) −1 Rcostello talk contribs (→‎"Pick and Place" Methodology)
     
08:12 (cur | prev) +110 Rcostello talk contribs (→‎References)
     
08:11 (cur | prev) +299 Rcostello talk contribs (→‎"Pick and Place" Methodology)
     08:02 Upload log Rcostello talk contribs uploaded File:Pick and Place.mp4

23 April 2024

     15:33  "Pick and Place" Assembly of Parts Using PDMS - Amy Lim, Rylie Costello‎‎ 6 changes history +837 [Rcostello‎ (6×)]
     
15:33 (cur | prev) +1 Rcostello talk contribs (→‎"Pick and Place" for Microfluidics)
     
15:33 (cur | prev) +203 Rcostello talk contribs (→‎References)
     
15:31 (cur | prev) −2 Rcostello talk contribs (→‎"Pick and Place" for Microfluidics)
     
15:29 (cur | prev) −474 Rcostello talk contribs (→‎References)
     
15:29 (cur | prev) +845 Rcostello talk contribs (→‎MEMS Devices)
     
15:14 (cur | prev) +264 Rcostello talk contribs (→‎"Pick and Place" for Microfluidics)
     11:58  BioMicroCenter:People‎‎ 2 changes history +30 [Lttran‎ (2×)]
     
11:58 (cur | prev) −4 Lttran talk contribs (→‎BioMicro Center Staff)
     
11:49 (cur | prev) +34 Lttran talk contribs (→‎BioMicro Center Staff)
     11:46 Upload log Lttran talk contribs uploaded File:SKR BMC.jpg

22 April 2024

     19:28  "Pick and Place" Assembly of Parts Using PDMS - Amy Lim, Rylie Costello‎‎ 2 changes history −2 [Rcostello‎ (2×)]
     
19:28 (cur | prev) −2 Rcostello talk contribs (→‎Nanowires)
     
19:26 (cur | prev) 0 Rcostello talk contribs (→‎Biology-Inspired Solution)
     19:01  Microfluidic Sensing- Microfluidic Biosensors- Xiao Fan‎‎ 13 changes history +131 [Khiemle‎ (13×)]
     
19:01 (cur | prev) +14 Khiemle talk contribs (→‎Microfluidic immunosensors)
     
19:00 (cur | prev) +7 Khiemle talk contribs (→‎DNA-based microfluidic biosensors)
     
19:00 (cur | prev) +18 Khiemle talk contribs (→‎Microfluidic immunosensors)
     
18:59 (cur | prev) +18 Khiemle talk contribs (→‎Microfluidic immunosensors)
     
18:58 (cur | prev) −2 Khiemle talk contribs (→‎DNA-based microfluidic biosensors)
     
18:58 (cur | prev) +2 Khiemle talk contribs (→‎Enzyme-based microfluidic biosensors)
     
18:58 (cur | prev) +1 Khiemle talk contribs (→‎Enzyme-based microfluidic biosensors)
     
18:58 (cur | prev) −9 Khiemle talk contribs (→‎Enzyme-based microfluidic biosensors)
     
18:57 (cur | prev) −40 Khiemle talk contribs (→‎DNA-based microfluidic biosensors)
     
18:57 (cur | prev) +2 Khiemle talk contribs (→‎DNA-based microfluidic biosensors)
     
18:56 (cur | prev) +34 Khiemle talk contribs (→‎DNA-based microfluidic biosensors)
     
18:56 (cur | prev) +86 Khiemle talk contribs (→‎Microfluidic immunosensors)
     
18:54 (cur | prev) 0 Khiemle talk contribs (→‎Enzyme-based microfluidic biosensors)



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25 April 2024

     00:22  The paper that launched microfluidics - Xi Ning‎‎ 7 changes history +4,723 [Xning098‎ (7×)]
     
00:22 (cur | prev) −97 Xning098 talk contribs
     
00:18 (cur | prev) +14 Xning098 talk contribs (→‎Summary)
     
00:11 (cur | prev) +1 Xning098 talk contribs (→‎Introduction)
     
00:11 (cur | prev) +1 Xning098 talk contribs (→‎Introduction)
     
00:11 (cur | prev) 0 Xning098 talk contribs (→‎References)
     
00:08 (cur | prev) +4 Xning098 talk contribs (→‎Significance)
     
00:07 (cur | prev) +4,800 Xning098 talk contribs

24 April 2024

     22:50  WAKNA:Basics‎‎ 8 changes history +610 [Berthold Drexler‎ (8×)]
     
22:50 (cur | prev) +136 Berthold Drexler talk contribs (→‎Hier finden Sie Literatur für Einsteiger:innen in das Gebiet der Neuroanästhesie)
     
22:47 (cur | prev) +238 Berthold Drexler talk contribs (→‎Neuromonitoring allgemein)
     
22:33 (cur | prev) +151 Berthold Drexler talk contribs (→‎Hier finden Sie Literatur für Einsteiger:innen in das Gebiet der Neuroanästhesie)
     
22:31 (cur | prev) 0 Berthold Drexler talk contribs (→‎Grundlagen, Physiologie & Pharmakologie)
     
22:30 (cur | prev) +1 Berthold Drexler talk contribs (→‎Grundlagen, Physiologie & Pharmakologie)
     
22:30 (cur | prev) +313 Berthold Drexler talk contribs (→‎Grundlagen, Physiologie & Pharmakologie)
     
06:23 (cur | prev) +192 Berthold Drexler talk contribs (→‎Hier finden Sie Literatur für Einsteiger:innen in das Gebiet der Neuroanästhesie)
     
06:21 (cur | prev) −421 Berthold Drexler talk contribs (→‎Sonstige)
     18:35  User:Yanbin Huang‎‎ 2 changes history +25 [Yanbin Huang‎ (2×)]
     
18:35 (cur | prev) +13 Yanbin Huang talk contribs (→‎Peer-Reviewed Publications)
     
18:34 (cur | prev) +12 Yanbin Huang talk contribs (→‎Peer-Reviewed Publications)
     17:49  Hu‎‎ 2 changes history +28 [Hugangqing‎ (2×)]
     
17:49 (cur | prev) +18 Hugangqing talk contribs
     
17:48 (cur | prev) +10 Hugangqing talk contribs
     08:14  "Pick and Place" Assembly of Parts Using PDMS - Amy Lim, Rylie Costello‎‎ 6 changes history +394 [Rcostello‎ (6×)]
     
08:14 (cur | prev) +1 Rcostello talk contribs (→‎"Pick and Place" Methodology)
     
08:13 (cur | prev) −14 Rcostello talk contribs (→‎"Pick and Place" Methodology)
     
08:12 (cur | prev) −1 Rcostello talk contribs (→‎"Pick and Place" Methodology)
     
08:12 (cur | prev) −1 Rcostello talk contribs (→‎"Pick and Place" Methodology)
     
08:12 (cur | prev) +110 Rcostello talk contribs (→‎References)
     
08:11 (cur | prev) +299 Rcostello talk contribs (→‎"Pick and Place" Methodology)
     08:02 Upload log Rcostello talk contribs uploaded File:Pick and Place.mp4

23 April 2024

     15:33  "Pick and Place" Assembly of Parts Using PDMS - Amy Lim, Rylie Costello‎‎ 6 changes history +837 [Rcostello‎ (6×)]
     
15:33 (cur | prev) +1 Rcostello talk contribs (→‎"Pick and Place" for Microfluidics)
     
15:33 (cur | prev) +203 Rcostello talk contribs (→‎References)
     
15:31 (cur | prev) −2 Rcostello talk contribs (→‎"Pick and Place" for Microfluidics)
     
15:29 (cur | prev) −474 Rcostello talk contribs (→‎References)
     
15:29 (cur | prev) +845 Rcostello talk contribs (→‎MEMS Devices)
     
15:14 (cur | prev) +264 Rcostello talk contribs (→‎"Pick and Place" for Microfluidics)
     11:58  BioMicroCenter:People‎‎ 2 changes history +30 [Lttran‎ (2×)]
     
11:58 (cur | prev) −4 Lttran talk contribs (→‎BioMicro Center Staff)
     
11:49 (cur | prev) +34 Lttran talk contribs (→‎BioMicro Center Staff)
     11:46 Upload log Lttran talk contribs uploaded File:SKR BMC.jpg

22 April 2024

     19:28  "Pick and Place" Assembly of Parts Using PDMS - Amy Lim, Rylie Costello‎‎ 2 changes history −2 [Rcostello‎ (2×)]
     
19:28 (cur | prev) −2 Rcostello talk contribs (→‎Nanowires)
     
19:26 (cur | prev) 0 Rcostello talk contribs (→‎Biology-Inspired Solution)
     19:01  Microfluidic Sensing- Microfluidic Biosensors- Xiao Fan‎‎ 13 changes history +131 [Khiemle‎ (13×)]
     
19:01 (cur | prev) +14 Khiemle talk contribs (→‎Microfluidic immunosensors)
     
19:00 (cur | prev) +7 Khiemle talk contribs (→‎DNA-based microfluidic biosensors)
     
19:00 (cur | prev) +18 Khiemle talk contribs (→‎Microfluidic immunosensors)
     
18:59 (cur | prev) +18 Khiemle talk contribs (→‎Microfluidic immunosensors)
     
18:58 (cur | prev) −2 Khiemle talk contribs (→‎DNA-based microfluidic biosensors)
     
18:58 (cur | prev) +2 Khiemle talk contribs (→‎Enzyme-based microfluidic biosensors)
     
18:58 (cur | prev) +1 Khiemle talk contribs (→‎Enzyme-based microfluidic biosensors)
     
18:58 (cur | prev) −9 Khiemle talk contribs (→‎Enzyme-based microfluidic biosensors)
     
18:57 (cur | prev) −40 Khiemle talk contribs (→‎DNA-based microfluidic biosensors)
     
18:57 (cur | prev) +2 Khiemle talk contribs (→‎DNA-based microfluidic biosensors)
     
18:56 (cur | prev) +34 Khiemle talk contribs (→‎DNA-based microfluidic biosensors)
     
18:56 (cur | prev) +86 Khiemle talk contribs (→‎Microfluidic immunosensors)
     
18:54 (cur | prev) 0 Khiemle talk contribs (→‎Enzyme-based microfluidic biosensors)