User:Alexander.wong/sandbox

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New Layout 2 --Anthony Lazzaro 19:31, 1 September 2007 (EDT) Alex's I've done tree and so I'd thought i have a go at front page



Breaking News !!


  • 28 Aug 2007 - 'Infector Modelling'
  • 15 Aug 2007 - 'We have vesicles!!'
News Archive link

INFORMATION At Base Camp


INFORMATION In the Wet Lab


visitors For our Visitors


Location of our visitors

Imperial College London iGEM'07 Projects

Cell Free Systems


INFORMATION Cell By Date INFORMATION Infector Detector

Recent Changes



People The Team


The Imperial iGEM team consists of ten undergraduate students working fulltime during summer 2007 on engineering a biological system. In addition, we have a number of graduate student and faculty advisors.

Undergraduates

Advisors

  • Prof Richard Kitney
  • Prof Paul Freemont
  • Duo Lu
  • Kirsten Jensen
  • Matthieu Bultelle
  • Vincent Rouilly
Acknowledgments

Entertainment Entertainment



In partnership with

GeneART









Layout for New iGEM page



Breaking News !!


  • 28 Aug 2007 - 'Infector Modelling'
  • 15 Aug 2007 - 'We have vesicles!!'
News Archive link

INFORMATION At Base Camp


INFORMATION In the Wet Lab


visitors For our Visitors


Location of our visitors

Imperial College London iGEM'07 Projects

Cell Free Systems


INFORMATION Cell By Date INFORMATION Infector Detector

Recent Changes



People The Team


The Imperial iGEM team consists of ten undergraduate students working fulltime during summer 2007 on engineering a biological system. In addition, we have a number of graduate student and faculty advisors.

Undergraduates

Advisors

  • Prof Richard Kitney
  • Prof Paul Freemont
  • Duo Lu
  • Kirsten Jensen
  • Matthieu Bultelle
  • Vincent Rouilly
Acknowledgments

Entertainment Entertainment



In partnership with

GeneART








Questions to ask the fluorometer Engineer on Friday, Aug 3

See if our plate reader has the ability to measure what you want to measure.

  • What are the classes of measurement? Can it do time-resolved fluorometry + Absorbance?
  • Delay time-resolve - 24 hour experiment; evaporation of solution over long-time courses?!
  • Temperature-regulation?
  • How do we subtract background values from data and convert from absorbance to OD600?

Talk about using the plate reader and creating protocols.

  • Excitation filters and Emission filters for GFP, acGFP and DsRed-Express?
  • Any manufacturer protocols for plate reader usage?
  • Well plate rows and columns? How is data arranged sequentially with the wells?
  • What kind of plates you need to order and use for our experiments?
  • Lamp energy and Noise signal ratios??

Talk about processing data

  • Export format?
  • How to export data from fluorometer?
  • Built-in data analysis tools?
  • Technical Support.


Experiment 1: Fluorescence vs extracellular [GFP] molecules

Known concentrations of GFP are diluted into a range of dilutions and are then mixed with cell lysate of a particular chassis.

Materials Required

Equipment

Reagents

Protocol

Experiment 2: Fluorescence vs Cultures of varying unknown intracellular [GFP]

Measuring the fluorescence of cultures of cells at set time intervals would measure a set of unknown intracellular [GFP].

Materials Required

Equipment

Reagents

Protocol

Experiment 3: Fluorescence vs Cultures of varying unknown extracellular [GFP]

Lysing samples of Experiment 2 will allow the relation of an unknwon intracellular [GFP] to that of extracellular [GFP].

Materials Required

Equipment

Reagents

Protocol

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