User:Alexander Boehme/Notebook/BioE140L

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(Project Description/Abstract)
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==Project Description/Abstract==
 
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* Place short description of project or notes regarding this project
 
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* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.
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AsbF-2 Synthon Construction File
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Mix oligios for asbF-2 synthon, do PCA1 protocol              (514 bp, pca1)
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PCR pca1 with asbF2-NewForward and asbF2-NewReverse by PCA2 protocol        (468 bp, pca2)
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Digest pca2 with EcoRI/BglII (yes, BglII), gp                (452+11+5 bp, pcr_dig)
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(get pre-digested vector from JCA of pBca9145-Bca1144  (EcoRI/BamHI, vect_dig)
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Ligate pcr_dig and vect_dig, transform, pick white colonies, map, sequence
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asbF2-NewForward
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ccaaaGAATTCCGTCTCATAGAAACCCATCC
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asbF2-NewReverse
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gactgAGATCTCGTCTCAGGTCTCAGGATCCAG
==Notes==
==Notes==

Revision as of 16:30, 5 March 2013

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AsbF-2 Synthon Construction File


Mix oligios for asbF-2 synthon, do PCA1 protocol (514 bp, pca1)

PCR pca1 with asbF2-NewForward and asbF2-NewReverse by PCA2 protocol (468 bp, pca2)

Digest pca2 with EcoRI/BglII (yes, BglII), gp (452+11+5 bp, pcr_dig)

(get pre-digested vector from JCA of pBca9145-Bca1144 (EcoRI/BamHI, vect_dig)

Ligate pcr_dig and vect_dig, transform, pick white colonies, map, sequence

asbF2-NewForward ccaaaGAATTCCGTCTCATAGAAACCCATCC

asbF2-NewReverse gactgAGATCTCGTCTCAGGTCTCAGGATCCAG

Notes

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