User:Alexander Boehme/Notebook/BioE140L/Entry Base

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(Autocreated Lab Notebook name=User:Alexander Boehme/Notebook/BioE140L/Entry Base, content from MediaWiki:EntryContentDefault)
Current revision (13:02, 12 March 2013) (view source)
(Entry title)
 
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==Entry title==
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PCA
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* Insert content here...
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38 uL ddH2O
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5 ul 10x expand buffer
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5 ul 2mM dNTPs
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1 ul oligo mixture (100uM total, mixture of oligos after combination of 100uM stocks)
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0.75 ul Expand polymerase
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Program (can run JCA/PCA1)
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__NOTOC__
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2 min initial denature at 94oC
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30 sec denature at 94oC
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30 sec anneal at 55oC [This should be the overlap temp of your oligos - vary as needed]
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30 sec extension at 72oC
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repeat 2-4 30 times total

Current revision

Project name Main project page

PCA 38 uL ddH2O 5 ul 10x expand buffer 5 ul 2mM dNTPs 1 ul oligo mixture (100uM total, mixture of oligos after combination of 100uM stocks) 0.75 ul Expand polymerase Program (can run JCA/PCA1) 2 min initial denature at 94oC 30 sec denature at 94oC 30 sec anneal at 55oC [This should be the overlap temp of your oligos - vary as needed] 30 sec extension at 72oC repeat 2-4 30 times total

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