User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/08/28: Difference between revisions

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=====Stock Solutions=====
=====Stock Solutions=====
Concentration of Gold Solution (HAuCl<sub>4</sub>*3H<sub>2</sub>O) = .245mM <br.>
Concentration of Gold Solution (HAuCl<sub>4</sub>*3H<sub>2</sub>O) = .245mM <br.>
Concentration of Sodium Citrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>*2H<sub>2</sub>O) = 1.01%
Concentration of Sodium Citrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>*2H<sub>2</sub>O) = 1.01% <br.>
Note, 1.5ml of sodium citrate was used instead of 3ml.  
=====Amount of Stock Solution Used=====
Note, 1.5ml of sodium citrate was used instead of 3ml. <br.>
Procedure was Followed to completion. <br.>
Final Amount of Solution after Procedure was 25.5ml. <br.>
UV-Vis Measurements were taken with a sample of solution that was a 10X dilution. As a result, final concentrations found must be augmented to fit this change.  
===Data===
===Data===

Revision as of 09:45, 31 August 2013

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

To prepare gold nanoparticles using two separate methods. Procedure taken from Dr. Matt Hartings, found here.

Procedure

BSA-AuNP

Stock Solutions

Concentration of Gold Solution (HAuCl4*3H2O) = 2.54mM <br.> Concentration of BSA = 15.6μM <br.>

Amount of Stock Solution Used

1ml of gold solution is used to prepare the gold nano particles, thus .00254 milli moles of Gold are present.<br.> In order to have 90 times less BSA than gold we need 2.82*10^5 milli moles of BSA.<br.> As a result, 1.809 ml of BSA is needed.

Citrate-AuNP

Stock Solutions

Concentration of Gold Solution (HAuCl4*3H2O) = .245mM <br.> Concentration of Sodium Citrate (Na3C6H5O7*2H2O) = 1.01% <br.>

Amount of Stock Solution Used

Note, 1.5ml of sodium citrate was used instead of 3ml. <br.> Procedure was Followed to completion. <br.> Final Amount of Solution after Procedure was 25.5ml. <br.> UV-Vis Measurements were taken with a sample of solution that was a 10X dilution. As a result, final concentrations found must be augmented to fit this change.

Data
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