User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/08/28: Difference between revisions

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*Absorbance at 450 nm = .091 AU<br.>
*Absorbance at 450 nm = .091 AU<br.>
*Using the reference above the particle diameter is 12 nm and the molar absorptivity is 1.09*10^8<br.>
*Using the reference above the particle diameter is 12 nm and the molar absorptivity is 1.09*10^8<br.>
*Using Beers Law and the reference above the calculated concentration of the diluted sample is 0.834nM<br.>
*The final concentration of the solution is 8.34nM

Revision as of 18:44, 31 August 2013

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Objective

To prepare gold nanoparticles using two separate methods. Procedure taken from Dr. Matt Hartings, found here.

Procedure

BSA-AuNP

Stock Solutions
  • Concentration of Gold Solution (HAuCl4*3H2O) = 2.54mM <br.>
  • Concentration of BSA = 15.6μM <br.>
Amount of Stock Solution Used
  • 1ml of gold solution is used to prepare the gold nano particles, thus .00254 milli moles of Gold are present.<br.>

In order to have 90 times less BSA than gold we need 2.82*10^5 milli moles of BSA.<br.>

  • As a result, 1.809 ml of BSA is needed.

Citrate-AuNP

Stock Solutions
  • Concentration of Gold Solution (HAuCl4*3H2O) = .245mM <br.>
  • Concentration of Sodium Citrate (Na3C6H5O7*2H2O) = 1.01% <br.>
Amount of Stock Solution Used
  • Note, 1.5ml of sodium citrate was used instead of 3ml. <br.>

Procedure was followed as written to completion. <br.>

After Synthesis of Gold Nanoparticles
  • Final Amount of Solution = 25.5ml <br.>

UV-Vis Measurements were taken with a sample of solution that was a 10X dilution. As a result, final concentrations found must be augmented to fit this change.

Data

<br.>

  • Wavelength at Peak = 518.5nm<br.>
  • Absorbance at Peak = 0.143 AU<br.>

In order to analyze the data the following reference was utilized, 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra'<br.> In order to find the concentration of the solution the Absorbance at 45onm needs to be known.<br.>

  • Absorbance at 450 nm = .091 AU<br.>
  • Using the reference above the particle diameter is 12 nm and the molar absorptivity is 1.09*10^8<br.>
  • Using Beers Law and the reference above the calculated concentration of the diluted sample is 0.834nM<br.>
  • The final concentration of the solution is 8.34nM
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