User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/08/28: Difference between revisions
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[[Image:AuNP's_Citrate_08-28-13.png]]<br.> | [[Image:AuNP's_Citrate_08-28-13.png]]<br.> | ||
*Wavelength at Peak = 518.5nm<br.> | *Wavelength at Peak = 518.5nm<br.> | ||
*Absorbance at Peak = 0.143 AU<br.> | *Absorbance at Peak = 0.143 AU (Note this value accounts for the signal due to water)<br.> | ||
In order to analyze the data the following reference was utilized, [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra']<br.> | In order to analyze the data the following reference was utilized, [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra']<br.> | ||
In order to find the concentration of the solution the Absorbance at 450nm needs to be known.<br.> | In order to find the concentration of the solution the Absorbance at 450nm needs to be known.<br.> |
Revision as of 17:03, 1 September 2013
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ObjectiveTo prepare gold nanoparticles using two separate methods. Procedure taken from Dr. Matt Hartings, found here. ProcedureBSA-AuNPStock Solutions
Amount of Stock Solution Used
In order to have 90 times less BSA than gold we need 2.82*10^5 milli moles of BSA.<br.>
Citrate-AuNPStock Solutions
Amount of Stock Solution Used
Procedure was followed as written to completion. <br.> After Synthesis of Gold Nanoparticles
UV-Vis Measurements were taken with a sample of solution that was a 10X dilution. This was done due to the linear relationship that forms with absorbances under the value of 1. As a result, final concentration found must be augmented to fit this change. Data
In order to analyze the data the following reference was utilized, 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra'<br.> In order to find the concentration of the solution the Absorbance at 450nm needs to be known.<br.>
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