User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/08/28: Difference between revisions
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*Using Beers Law and the reference above the calculated concentration of the diluted sample is 0.834nM<br.> | *Using Beers Law and the reference above the calculated concentration of the diluted sample is 0.834nM<br.> | ||
*Taking into account the dilution the final concentration of the Gold AuNP's prepared using Citrate is 8.34nM | *Taking into account the dilution the final concentration of the Gold AuNP's prepared using Citrate is 8.34nM | ||
[[User:Matt Hartings|Matt Hartings]] By your measurements (final volume of your solution), what concentration of gold (not AuNPs just gold) do you expect these samples to have? |
Revision as of 17:36, 11 September 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo prepare gold nanoparticles using two separate methods. Procedure taken from Dr. Matt Hartings, found here. ProcedureBSA-AuNPStock Solutions
Amount of Stock Solution Used
In order to have 90 times less BSA than gold we need 2.82*10^5 milli moles of BSA.<br.>
Citrate-AuNPStock Solutions
Amount of Stock Solution Used
Procedure was followed as written to completion. <br.> After Synthesis of Gold Nanoparticles
UV-Vis Measurements were taken with a sample of solution that was a 10X dilution. This was done due to the linear relationship that forms with absorbances under the value of 1. As a result, final concentration found must be augmented to fit this change. Data
In order to analyze the data the following reference was utilized, 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra'<br.> In order to find the concentration of the solution the Absorbance at 450nm needs to be known.<br.>
Matt Hartings By your measurements (final volume of your solution), what concentration of gold (not AuNPs just gold) do you expect these samples to have? |