User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/09/24

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(Autocreate 2013/09/24 Entry for User:Alexander_Cvitan/Notebook/Experimental_Biological_Chemistry_Lab)
Current revision (14:21, 30 September 2013) (view source)
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
 
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* Insert content here...
 
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==Objective==
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*Complete the procedure as outlined by Dr.Hartings [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24|here]].<br.>
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*Observe activity of pepsin and pepstatin in samples of hemoglobin. <br.>
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*Observe activity using UV-Vis after certain intervals of time.<br.>
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*At each measured interval take a sample of solution and stop the reaction to observe using SDS-PAGE. <br.>
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==Procedure==
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*Note that the hemoglobin had a concentration of 2nm in the pepsin solution.<br.>
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*Note that the hemoglobin had a concentration of 20nm in the pepstatin solution. <br.>
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==Data==
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*This graph represents the absorbance spectra of each hemoglobin and pepsin or hemoglobin and pepstatin solution at each time interval.
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*It is worth noting that it is believed that the pepsin used wasn't active. This is because their wasn't a drastic difference between samples including pepsin and pepstatin.
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[[Image:Sept_24_Pep's.png]]
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*Note that all of the samples were normalized by subtracting the signal of the buffer. Also the signals can be compared to the hemoglobin standard.
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Current revision

Biomaterials Design Lab Main project page
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Objective

  • Complete the procedure as outlined by Dr.Hartings here.
  • Observe activity of pepsin and pepstatin in samples of hemoglobin.
  • Observe activity using UV-Vis after certain intervals of time.
  • At each measured interval take a sample of solution and stop the reaction to observe using SDS-PAGE.

Procedure

  • Note that the hemoglobin had a concentration of 2nm in the pepsin solution.
  • Note that the hemoglobin had a concentration of 20nm in the pepstatin solution.

Data

  • This graph represents the absorbance spectra of each hemoglobin and pepsin or hemoglobin and pepstatin solution at each time interval.
  • It is worth noting that it is believed that the pepsin used wasn't active. This is because their wasn't a drastic difference between samples including pepsin and pepstatin.

Image:Sept_24_Pep's.png

  • Note that all of the samples were normalized by subtracting the signal of the buffer. Also the signals can be compared to the hemoglobin standard.
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