User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/09/24: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | |||
*Complete the procedure as outlined by Dr.Hartings [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24|here]].<br.> | |||
*Observe activity of pepsin and pepstatin in samples of hemoglobin. <br.> | |||
*Observe activity using UV-Vis after certain intervals of time.<br.> | |||
*At each measured interval take a sample of solution and stop the reaction to observe using SDS-PAGE. <br.> | |||
==Procedure== | |||
*Note that the hemoglobin had a concentration of 2nm in the pepsin solution.<br.> | |||
*Note that the hemoglobin had a concentration of 20nm in the pepstatin solution. <br.> | |||
==Data== | |||
*This graph represents the absorbance spectra of each hemoglobin and pepsin or hemoglobin and pepstatin solution at each time interval. | |||
*It is worth noting that it is believed that the pepsin used wasn't active. This is because their wasn't a drastic difference between samples including pepsin and pepstatin. | |||
[[Image:Sept_24_Pep's.png]] | |||
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*Note that all of the samples were normalized by subtracting the signal of the buffer. Also the signals can be compared to the hemoglobin standard. | |||
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Latest revision as of 23:23, 26 September 2017
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Objective
Procedure
Data
|
- Note that all of the samples were normalized by subtracting the signal of the buffer. Also the signals can be compared to the hemoglobin standard.