User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/09/25: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
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* Well 11. Dr.Fox's Sample<br.>
* Well 11. Dr.Fox's Sample<br.>
* Well 12. 100 times diluted hemoglobin and pepsin<br.>
* Well 12. 100 times diluted hemoglobin and pepsin<br.>
**Note that we used well 12 of Carly's group to analyze 100 times diluted hemoglobin and pepstatin<br.>
**Note that a mistake was made while loading, the gel doesn't have a standard for 100x diluted hemoglobin with pepstatin. <br.>


*Also Note that the overnight pepsin and pepstatin samples were viewed using UV-Vis today, as outlined in the procedure. Data is presented; however, in yesterdays writeup.
*Also Note that the overnight pepsin and pepstatin samples were viewed using UV-Vis today, as outlined in the procedure. Data is presented; however, in yesterdays writeup.


==Data==
==Data==
*Please note that some of the contents of well 11 might have leaked into well 10.  
*Please note that some of the contents of well 11 leaked into well 10.  
*Wells are labeled left to right.  
*Wells are labeled left to right.  
[[Image:Sept_25_gel.png]]
[[Image:Sept_25_gel.png]]
 
*Based off of these results it becomes apparent that the pepsin most likely wasn't active. Looking at the bands their really isn't any visual difference in band intensity except for the standard. This test should be run again with another sample of pepsin to ensure that that was the problem.<br.>
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__NOTOC__
__NOTOC__

Latest revision as of 23:23, 26 September 2017

Biomaterials Design Lab Main project page
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Objective

  • Complete the procedure outlined by Dr.Hartings here.
  • Run SDS-PAGE samples that were prepared yesterday and run densitometry of the bands to determine the amount of protein present.

Procedure

  • The gel was loaded as follows,<br.>
  • Well 1. 100 times diluted hemoglobin and dye<br.>
  • Well 2. Pepsin Time 1<br.>
  • Well 3. Pepsin Time 2<br.>
  • Well 4. Pepsin Time 3<br.>
  • Well 5. Pepsin Time 4<br.>
  • Well 6. Pepstatin Time 1<br.>
  • Well 7. Pepstatin Time 2<br.>
  • Well 8. Pepstatin Time 3<br.>
  • Well 9. Pepstatin Time 4<br.>
  • Well 10. 100 Times diluted hemoglobin and dye<br.>
  • Well 11. Dr.Fox's Sample<br.>
  • Well 12. 100 times diluted hemoglobin and pepsin<br.>
    • Note that a mistake was made while loading, the gel doesn't have a standard for 100x diluted hemoglobin with pepstatin. <br.>
  • Also Note that the overnight pepsin and pepstatin samples were viewed using UV-Vis today, as outlined in the procedure. Data is presented; however, in yesterdays writeup.

Data

  • Please note that some of the contents of well 11 leaked into well 10.
  • Wells are labeled left to right.

  • Based off of these results it becomes apparent that the pepsin most likely wasn't active. Looking at the bands their really isn't any visual difference in band intensity except for the standard. This test should be run again with another sample of pepsin to ensure that that was the problem.<br.>


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