User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/09: Difference between revisions
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==Data== | ==Data== | ||
===Spectra of ADA Conversion of Adenosine to Inosine=== | |||
* This graph represents the conversion of Adenosine to Inosine using ADA and inhibitor. Shown in the graph, there is a shift in the spectra which were recorded every 15 sec for 10 min. The shift; however is less prominent than it was yesterday without the inhibitor. | * This graph represents the conversion of Adenosine to Inosine using ADA and inhibitor. Shown in the graph, there is a shift in the spectra which were recorded every 15 sec for 10 min. The shift; however is less prominent than it was yesterday without the inhibitor. | ||
* Overall it was observed that the adenosine peak shrunk more slowly overtime, and everything appeared to shift to the right. | * Overall it was observed that the adenosine peak shrunk more slowly overtime, and everything appeared to shift to the right. | ||
[[Image:Screen_shot_2013-10-19_at_8.38.29_PM.png]] | [[Image:Screen_shot_2013-10-19_at_8.38.29_PM.png]] | ||
*At peak 250 nm; the molar absorptivity of adenosine and inosine are 10224 (1/M*cm) and 11007 (1/M*cm) respectively. | *At peak 250 nm; the molar absorptivity of adenosine and inosine are 10224 (1/M*cm) and 11007 (1/M*cm) respectively. | ||
*At Peak 260 nm; the molar absorptivity of adenosine and inosine are 14025 (1/M*cm) and 6630 (1/M*cm) respectively. | *At Peak 260 nm; the molar absorptivity of adenosine and inosine are 14025 (1/M*cm) and 6630 (1/M*cm) respectively. | ||
===Concentration vs. Time for ADA Conversion of Adenosine to Inosine=== | |||
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Revision as of 17:41, 19 October 2013
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Objective
Procedure
DataSpectra of ADA Conversion of Adenosine to Inosine
Concentration vs. Time for ADA Conversion of Adenosine to Inosine |