User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/09: Difference between revisions

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*At peak 250 nm; the molar absorptivity of adenosine and inosine are 10224 (1/M*cm) and 11007 (1/M*cm) respectively.
*At peak 250 nm; the molar absorptivity of adenosine and inosine are 10224 (1/M*cm) and 11007 (1/M*cm) respectively.
*At Peak 260 nm; the molar absorptivity of adenosine and inosine are 14025 (1/M*cm) and 6630 (1/M*cm) respectively.
*At Peak 260 nm; the molar absorptivity of adenosine and inosine are 14025 (1/M*cm) and 6630 (1/M*cm) respectively.
===Concentration vs. Time for ADA Conversion of Adenosine to Inosine===
===Concentration vs. Time for ADA Conversion of Adenosine to Inosine using an Inhibitor===


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Revision as of 17:52, 19 October 2013

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  • To complete the procedure outline by Dr.Hartings HERE.
  • We hope to outline the kinetics of the conversion of a solution of Adenosine to Inosine using ADA and a EHNA inhibitor.

Procedure

  • Measured .1065 g Adenosine and added it to 10 ml buffer to create approx 40 mM. 3 micro-liters 40mM in 2996 micro-liters of buffer and 4 micro-liter of EHNA inhibitor.<br.>
  • In Dr.Hartings lab we added 30 ul of ADA after data was recorded 5 times. Data was recorded every 15 sec for 10 min.<br.>
  • Dilutions were made with 50mM Phosphate buffer pH 74 <br.>
  • ADA Stock Solution 0.11 units/mL ADA<br.>
  • EHNA Stock 3μM <br.>

Data

Spectra of ADA Conversion of Adenosine to Inosine

  • This graph represents the conversion of Adenosine to Inosine using ADA and inhibitor. Shown in the graph, there is a shift in the spectra which were recorded every 15 sec for 10 min. The shift; however is less prominent than it was yesterday without the inhibitor.
  • Overall it was observed that the adenosine peak shrunk more slowly overtime, and everything appeared to shift to the right.

  • At peak 250 nm; the molar absorptivity of adenosine and inosine are 10224 (1/M*cm) and 11007 (1/M*cm) respectively.
  • At Peak 260 nm; the molar absorptivity of adenosine and inosine are 14025 (1/M*cm) and 6630 (1/M*cm) respectively.

Concentration vs. Time for ADA Conversion of Adenosine to Inosine using an Inhibitor


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