User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/09: Difference between revisions
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==Procedure== | ==Procedure== | ||
*Measured .1065 g Adenosine and added it to 10 ml buffer to create approx 40 mM. 3 micro-liters 40mM | *Measured .1065 g Adenosine and added it to 10 ml buffer to create approx 40 mM adenosine solution. 3 micro-liters 40mM adenosine was added to 2996 micro-liters of buffer and 4 micro-liter of EHNA inhibitor to create a 40uM solution of adenosine.<br.> | ||
*In Dr.Hartings lab we added 30 ul of ADA after data was recorded 5 times. Data was recorded every 15 sec for 10 min.<br.> | *In Dr.Hartings lab we added 30 ul of ADA after data was recorded 5 times. Data was recorded every 15 sec for 10 min.<br.> | ||
* Dilutions were made with 50mM Phosphate buffer pH 74 <br.> | * Dilutions were made with 50mM Phosphate buffer pH 74 <br.> |
Revision as of 18:26, 19 October 2013
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Objective
Procedure
DataSpectra of ADA Conversion of Adenosine to Inosine
Concentration vs. Time for ADA Conversion of Adenosine to Inosine using an Inhibitor
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